Sensitive and reproducible flow injection spectrophotometric method for determination of fluoxetine in pharmaceutical preparation has been developed. The method is based on base hydrolysis of the drug. The hydrolyzed product methylamine is reacted with sodium nitroprusside. The absorbance of the resulting colored product was measured spectrophtometrically at 510 nm using flow injection method. ...

A new high-performance liquid chromatographic method is described for the determination of bisoprolol in human plasma. The proposed method was based on the derivatization of bisoprolol with 4-chloro-7-nitro-2,1,3-benzoxadiazole in borate buffer at pH 9.5 to yield a fluorescent product. Chromatographic separation of bisoprolol was achieved by using isocratic elution at a flow rate of 1.2 mL/min ...

Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high speci...

A simple and rapid micellar electrokinetic chromatography (MEKC) method was developed for the analysis of an antiviral drug, oseltamivir, and its hydrolyzed product in Tamiflu capsules. Background electrolytes consisted of boric acid 10 mM, pH 10, and sodium dodecyl sulphate (SDS) 40 mM. The limit of detection (LOD) and limit of quantification (LOQ) of oseltamivir were 1.7 and 8.0 microg/ml, re...

A selective and simple spectrophotometric method has been developed for the determination of phenylpropanolamine HCl (PPA) in its dosage forms. The method was based on the formation of a colored N-vinyl chlorobenzoquinone derivative of PPA through its reaction with 2,3,5,6-tetrachloro-1,4-benzoquinone in presence of acetaldehyde. The colored product exhibits maximum absorbance at 650 nm. Differ...

In a newsvendor inventory system, demand observations often get right censored when there are lost sales and no backordering. Demands for newsvendor-type products are often forecasted from censored observations. The Kaplan-Meier product limit estimator is the well-known nonparametric method to deal with censored data, but it is undefined beyond the largest observation if it is censored. To addr...

Television (TV) Commercial program contains important product information that displayed only in seconds. People who need that information has no insufficient time for noted it, even just for reading that information. This research work focus on automatically detect text and extract important information from a TV commercial to provide information in real time and for video indexing. We propose...

A highly selective and sensitive enzymatic method for the quantitative determination of L-arginine (Arg) has been developed. The method is based on the use of recombinant bacterial arginine deiminase (ADI) isolated from the cells of a recombinant strain Escherichia coli and o-phthalaldehyde (OPA) as a chemical reagent. Ammonia, the product of the enzymatic digestion of Arg by ADI, reacts with O...