Antibody to an estrogen inducible mouse uterine protein (Teng, C. T., Walker, M. P., Bhattacharyya, S. N., Klapper, D. G., DiAugustine, R. P., and McLachlan, J. A. (1986) Biochem. J. 240, 413-422) has been used to isolate cDNA to the messenger RNA. Analysis of the deduced primary structure and additional biochemical characterization indicates that the protein is lactotransferrin. An increase in...

1. Central dopaminergic pathways are possibly related to noradrenergic tone and may be important in the pathogenesis of essential hypertension. Basal plasma prolactin levels have been used as a marker of dopaminergic activity in the hypothalamus. The prolactin response to thyrotropin-releasing hormone (TRH) gives evidence of the dynamics of prolactin secretion. 2. To evaluate further the role o...

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A radioimmunoassay for primate prolactin has been developed, with [(131)I] monkey prolactin, and antibodies to monkey or human prolactin. The assay is specific for prolactin; human growth hormone, and human and monkey placental lactogen show no significant crossreaction. The assay is sensitive enough to measure prolactin concentrations in the sera of most humans studied. The concentration of pr...

Described is a two-chromatographic-step preparative-scale technique for the purification of human prolactin from a frozen pituitary homogenate. The method utilizes hydrophobic interaction chromatography on the mildly hydrophobic adsorbent phenyl-Sepharose CL-4B and anion-exchange chromatography on DEAE-cellulose in the presence of acetonitrile. Human prolactin was solubilized at pH10.0 after a ...

There is an inverse relation between zinc (Zn) intake and plasma prolactin in men and nonpregnant women. Whether a relation exists in lactating women is unknown, despite the potential consequences of perturbations in prolactin regulation on lactation performance. We examined the effects of low Zn intake on prolactin concentration, the prolactin regulatory pathway in the pituitary gland, and lac...

Prolactin is a dual function protein that functions both as a hormone as well as a cytokine (Ben-Jonathan et al., 1996). Prolactin is synthesized and secreted from pituitary gland and its primary role is to help initiate and maintain breast milk production in pregnant and nursing women (PRL prolactin). Prolactin is also synthesized and secreted from the cells of the immune system such as macrop...

The changes in the serum prolactin levels during 8 ovulatory cycles, 3 anovulatory cycles, 3 clomiphene treated cycles, 4 premarin treated cycles and 2 cycles which had just become pregnant were measured by our heterologous radioimmunoassay. Serum prolactin levels showed an increase in the ovulatory phase in 6 out of 8 ovulatory women and in the luteal phase in 5 out of 8 ovulatory women respec...

Bromocriptine (2-bromo-a-ergocryptine) inhibited prolactin release when added to primary cultures of dispersed pituitary glands from male rats. The total amount of prolactin (intracellular plus extracellular) was reduced to less than 20% of control values after 6 days. The amount of intracellular prolactin was increased in treated cultures and the total amount of hormone was the same in control...

The rate of tumor cell shedding into efferent tumor blood was measured in growing and regressing MTW9 rat mammary carcinomas. The hormone-dependent tumor, grown as an 'isoession was induced by reduction of mammotropin level in the host. Tumor cells were differentiated from normal leukocytes by indirect immunofluorescence. Growing tumors shed 3.2 x 10-6 and regressing tumors shed 4.1 x 10-6 cell...