Received 23 July 2008; received in revised form 11 November 2008; accepted 12 November 2008 Available online 24 November 2008 Upstream interactions of Escherichia coli RNA polymerase (RNAP) in an open promoter complex (RPo) formed at the PR and PRM promoters of bacteriophage λ have been studied by atomic force microscopy. We demonstrate that the previously described 30-nm DNA compaction observe...

Indoor wireless localization using Bluetooth Low Energy (BLE) beacons has attracted considerable attention after the release of the BLE protocol. In this paper, we propose an algorithm that uses the combination of channel-separate polynomial regression model (PRM), channel-separate fingerprinting (FP), outlier detection and extended Kalman filtering (EKF) for smartphone-based indoor localizatio...

Probabilistic Roadmap (PRM) planners have been used to generate paths for articulated robots for several years. By using random sampling techniques, PRM based planners are able to plot paths for robots with many degrees of freedom without needing to explore large parts of the search space that traditional planners would have to examine to create efficient paths. This has enabled them to be used...

We present, implement, and analyze a spectrum of closely-related planners, designed to gain insight into the relationship between classical grid search and probabilistic roadmaps (PRMs). Building on quasi-Monte Carlo sampling literature, we have developed deterministic variants of the PRM that use low-discrepancy and low-dispersion samples, including lattices. Classical grid search is extended ...

The DenV pre-membrane protein (prM) is a crucial chaperone for the viral envelope protein, preventing premature fusion with vesicles during viral export. prM molecules in immature particles are cleaved by host proteases, leading to mature fusogenic virions. Blockade of prM cleavage would restrict fusion and represents a novel druggable opportunity against DenV. We have thus established a cell-b...

Dengue virus infection is a common tropical disease which often occurs without being detected. These asymptomatic cases provide information in relation to the manifestation of immunological aspects. In this study, we developed an ELISA method to compare neutralizing effects of dengue prM and E antibodies between dengue patients and their asymptomatic household members. Recombinant D2 premembran...

The European Community is based on a series of treaties and legal decisions, which result from preliminary documents prepared long before by different organizations and lobbies. The European union of medical specialists (Union européenne des médecins specialists [UEMS]) came into being in order to address the questions raised by European directives (e.g., free circulation of people and services...

concentration of a sample that gave an absorbance value within the linear range of the assay and was expressed relative to the absorbance/␮g of BSA (corresponding to the gradient of the calibration curve): Interference ϭ absorbance/␮g of aminoglycoside absorbance/␮g of BSA ϫ 100% The aminoglycosides gave negligible interference in the CBB assay (Ͻ0.2% relative to BSA) and failed to generate tur...

tires represent a critical spare part in mines. there is a shortage of medium and large tires. in addition, with increased mining activities and the creation of new mines, the demand for tires has increased significantly. thus, it is particularly important for mining engineers to identify tire characteristics and correctly manage the spare part inventory. spare parts management is critical from...