The bacterial metabolism of propylene proceeds by epoxidation to epoxypropane followed by a sequence of three reactions resulting in epoxide ring opening and carboxylation to form acetoacetate. Coenzyme M (2-mercaptoethanesulfonic acid) (CoM) plays a central role in epoxide carboxylation by serving as the nucleophile for epoxide ring opening and the carrier of the C(3) unit that is ultimately c...

The adsorption of propylene on clean and hydrogen-covered Pd(1 1 1) has been studied by using temperatureprogrammed desorption (TPD) and reflection-absorption infrared spectroscopy. Propylene adsorbs in a di-r configuration on clean Pd(1 1 1). At high exposures, it forms second and subsequent layers. Upon heating, propylene desorbs molecularly at 200 and 280 K and, in contrast to ethylene adsor...

DegP, a member of the HtrA family of proteins, conducts critical bacterial protein quality control by both chaperone and proteolysis activities. The regulatory mechanisms controlling these two distinct activities, however, are unknown. DegP activation is known to involve a unique mechanism of allosteric binding, conformational changes and oligomer formation. We have uncovered a novel role for t...

Catechin was oligomerized using free laccase and laccase-gum Arabic conjugate. The process of oligomerization was optimized with respect to solvent, ratio of solvent to buffer (0.2:10 to 1:10), pH of buffer (3-10), enzyme (575-18,400 U/mg) and substrate concentration (1-7mM). Maximum production of oligomer was observed in methanol at ratio 0.6:10 of methanol:buffer of pH 5 using 2300 U/mg of la...

The main goal of this study was to describe the method of the synthesis of the dodecyl-, tetradecyl-, hexadecyl- and octadecyl-propylene glycol emulsifiers in the presence of selected anionic and nonionic surfactants. Acyl propylene glycol emulsifiers were produced by esterification of propane-1,2-diol (propylene glycol, PG) with C12:0-C18:0 fatty acids in the presence of anionic sodium dodecyl...

Microtubule protein purified from porcine brain was fixed at low protein concentration with glutaraldehyde under conditions which maximize the relative concentration of the ring-shaped 30 S oligomer. Fixed oligomer was separated from glutaraldehyde and other protein species by column chromatography. The fixed, isolated oligomer was deposited on electron microscopy grids, dehydrated, and then cr...

Small heat shock proteins (sHSPs) make up a class of molecular chaperones broadly observed across organisms. Many sHSPs form large oligomers that undergo dynamic subunit exchange that is thought to play a role in chaperone function. Though remarkably heterogeneous, sHSP oligomers share three types of intermolecular interactions that involve all three defined regions of a sHSP: the N-terminal re...

Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by neurofibrillary tangles, synaptic impairments, and loss of neurons. Oligomers of β-amyloid (Aβ) are widely accepted as the main neurotoxins to induce oxidative stress and neuronal loss in AD. In this study, we discovered that fucoxanthin, a marine carotenoid with antioxidative stress properties, concentrat...

A new methacrylate monomer with two propylene glycol groups on the side chain, di(propylene glycol) methyl ether (diPGMA), was synthesised and homo- co-polymerised for first time.