Flax is an economic crop with a long history. It grown worldwide and mainly used for edible oil, industry, textiles. Here, we reported high-quality genome assembly “Neiya No. 9”, popular variety widely in China. Combining PacBio reads, Hi-C sequencing, genetic map previously, of 473.55 Mb was constructed, which covers ~94.7% the flax genome. These sequences were anchored onto 15 chromosomes. Th...

background: elongation factor-1, a conserved nuclear protein coding gene was used to identify iranian sandfly species. the phlebotomine sandfies are the vectors of the parasitic protozoan leishmania, the causative agents of leishmaniasis, in iran. methods: seven sets of primers were tried. pcr amplification of elongation factor-1 was successfully achieved for all 14 species of iranian sandfli...

Not all members of the human histone gene family are functional. We have isolated a human H2B pseudogene that contains alterations in the protein-coding sequences as well as in the 3' and 5' flanking sequences that preclude expression of a functional H2B histone protein. There are three modifications in the amino acid-coding region: a single-base deletion producing a frame shift, a single-base ...

The RNA 3 of rice stripe virus (RSV) isolate T was cloned and its nucleotide sequence was determined. The complete primary structure was found to consist of 2504 nucleotides. One putative open reading frame (ORF), between nucleotides 2412 and 1444 in the 5'-proximal region of the virus complementary-sense RNA, encoded a 322 amino acid protein with an Mr of 35,134 that was identified as the coat...

Plant lipid transfer proteins (LTPs) are members of the pathogenesis-related proteins (PR-14) and some of them exhibit activity against phytopathogenic fungi. To investigate whether rice LTP2 plays a role in antifungal activity, the coding region of an Iranian rice Ltp2 gene was cloned into expression vector pET24-d(+) and then expressed in Escherichia coli Rosetta strain (DE3). The potential a...

background: the hepatic secretion of apolipoprotein b (apob), containing lipoproteins, is known to be regulated by insulin, and the overproduction of these atherogenic lipoproteins occurs in insulin-resistant states. protein tyrosine phosphatase 1b (ptp-1b) is a key regulator of hepatic insulin signaling and is also upregulated in insulin resistance. we aimed to investigate the role of ptp-1b i...

We have prepared full-length DNA clones of the Abelson murine leukemia virus (A-MuLV) genome. A specific probe homologous to the central portion of the A-MuLV genome was prepared by nick translation of a subcloned restriction fraction from the cloned DNA. The probe was used to examine the genome structure of several A-MuLV variants. The conclusions are: (i) three viruses coding for Abelson-spec...

In animals, mitochondrial DNA (mtDNA) typically occurs as a single circular chromosome with 13 protein-coding genes and 22 tRNA genes. The various species of lice examined previously, however, have shown mitochondrial genome rearrangements with a range of chromosome sizes and numbers. Our research demonstrates that the mitochondrial genomes of two species of chewing lice found on pocket gophers...

• First mitogenome of subfamily Langiinae has been sequenced. All four currently recognized subfamilies were recovered as monophyletic. was the first to diverge within Sphingidae. Barbourion suggested be removed from Ambulycini, and placed in “Smerinthinae incertae sedis”. To date, a relatively complete classification Sphingidae (Lepidoptera) generated, but phylogeny family remains need fully r...