Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

The surfactant precipitation and recovery method (SPR method) consist of a step, that is protein-surfactant complex by the addition aqueous solution to protein solution, dissolution precipitate in polar organic solvent trace amount salt solution. When denaturant was not included, ratio lysozyme increased as AOT/lysozyme molar increased, after reaching 100%, decreased due redissolution precipita...

Infrared Spectroscopy has become a usual technique in studies of protein conformation. The use of modern instruments based on interferometric devices together with the application of Fourier-transform techniques makes possible to obtain high signal-to-noise ratio spectra. These advantages allow to apply resolution enhancement techniques such as deconvolution or derivation in order to derive inf...

SUMO modification of human thymine-DNA glycosylase facilitates the processing of base excision repair substrates by an unusual mechanism: while leaving the catalytic center unaffected, it induces product release by eliciting a conformational change in the enzyme.

In prion diseases, such as variant Creutzfeldt-Jakob disease normal cellular prion protein (PrPC), a largely alpha-helical structure is converted to an abnormal conformational isoform (PrPSc) that shows an increase in beta-sheet content. Similarly, the recombinant form of PrPC (ralpha-PrP) can be converted to a conformation dominated by beta-sheet (rbeta-PrP) by reduction and mild acidification...

The NF-κB family of transcription factor is a central mediator immune and imflammatory responses. functions as dimer recognize DNA through the two N-terminal domains (NTDs). Previous hydrogen-deuterium exchange mass spectrometry, stopped-flow experiments molecular dynamics simulations suggested NTDs can undergo relative motions which plays an important role in kinetics association dissociation....

Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

The interaction between the solute and the solsent molecules play a crucial role in understanding the various molecular processes involved in chemistry and biochemistry, so in this work the potential energy of active site of azurin have been calculated in solvent by the Monte Carlo simulation. In this paper we present quantitative results of Monte Carlo calculations of potential energies of ...