Purpose: To investigate the effect of excisanin A on human hepatocellular carcinoma cells as well to elucidate its mechanism action.
 Methods: Molecular docking was used determine binding characteristics HIF-1α protein. The transcriptional activation and viability were assessed using Luciferase reporter MTT assay. protein in nucleus assayed western blot immunofluorescence. VEGF mRNA levels...

Polymer–Protein Hybrids Polymer–protein hybrids provide a new route for protein stabilization in harsh environments. In article number 2201809, Michael A. Webb, Adam J. Gormley, and co-workers demonstrate strategy combining automated polymer synthesis machine learning to design copolymers tailored enhance the stability of target proteins. This may overcome challenges accelerate development asso...

Non-radioactive assays based on incorporation of puromycin into newly synthesized proteins and subsequent detection using anti-puromycin antibodies have been previously reported and well-validated. To develop a moderate- to high-throughput assay, an adaptation is here described wherein cells are puromycin-labeled followed by simultaneously probing puromycin-labeled proteins and a reference prot...

The teaching of themes considered to be vast abstraction, such as Protein Synthesis, is a challenge for teachers, since it needs pedagogical practices that bring the subject closer reality students and at same time are effective. Emergency distance education proposed developing activities adapted virtual environment, allowing development new methods, case with use gamification, which inserts ga...

objective: angiogenesis a process that results in neo-vascularization is an essential stage in growth of solid tumors and the formation of metastases. vascular endothelial growth factor (vegf) and its receptors, vegfr1 (flt-1) and vegfr2 (kdr/flk-1), are the major regulators for tumor angiogenesis. recent studies showed that second domain of vegfr-1is a key factor for vegf/vegfr-1 interaction. ...

The engineering of proteins can illuminate their biological function and improve their performance in a variety of applications. Within the past decade, methods have been developed that facilitate the ability of chemists to manipulate proteins in a controlled manner. Here, we present the traceless Staudinger ligation as a strategy for the convergent chemical synthesis of proteins. This reaction...

background and aims: to evaluate the effect of temperature on wheat streak mosaic virus (wsmv) resistance phenotype, through total protein, phenol, and peroxidase activity in bread wheat, a factorial experiment was conducted using adl-cross (resistant) and marvdasht (susceptible) cultivars. materials and methods: results showed that incubation at 32c changed the gene expression for resistance ...

an efficient synthesis of polyhydroquinoline derivatives is achieved via hantzsch condensation reaction between aldehydes, dimedone, ethyl acetoacetate and ammonium acetate in the presence of catalytic amount of sfhs in ethanol. this method gives remarkable advantages such as shorter reaction time, simple workup procedure and good to excellent yields. furthermore the catalyst can be recovered c...

Ovalbumin, as the major component of egg-white, is a globular, biocompatible, nontoxic and biodegradable phosphoglyco protein. This protein with the molecular weight of 44.5 kDa, contains 385 residues of amino acids with isoelectric point (pI) of 4.5. Many purification procedures have been reported for egg-white proteins such as gel permeation and anion exchange chromatography. In this study, w...