The recent development of high-throughput molecular genetics technologies has brought a major impact to bioin-formatics and systems biology. These technologies have made possible the measurement of the expression profiles of genes and proteins in a highly parallel and integrated fashion. The examination of the huge amounts of genomic and proteomic data holds the promise for understanding the co...

Proteomics is the large-scale study of the structure and function of proteins in complex biological sample. Such an approach has the potential value to understand the complex nature of the organism. Current proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. Advances in protein fractionation and labeling techni...

Despite worldwide efforts biomarker discovery by plasma proteomics was not successful so far. Several reasons for this failure are obvious. Mainly, proteome diversity is remarkable between different individuals and is caused by genetic, environmental and life style parameters. To recognize disease related proteins that could serve as potential biomarkers is only feasible by investigating a non ...

Due to the complex nature of the proteome, instrumentation and methods development for sample cleanup, fractionation, preconcentration, chromatographic separation and detection becomes urgent for the identification of peptides and proteins. Newly developed techniques and equipment for separation and detection, such as nanoHPLC and multidimensional HPLC for protein and peptide separation, enable...

A new peak picking algorithm for the analysis of mass spectrometric (MS) data is presented. It is independent of the underlying machine or ionization method, and is able to resolve highly convoluted and asymmetric signals. The method uses the multiscale nature of spectrometric data by first detecting the mass peaks in the wavelet-transformed signal before a given asymmetric peak function is fit...

background: transforming growth factor-beta (tgf-β) activation appears to be crucial for tissue injury in diabetic nephropathy (dn). fibromodulin, the small leucine-rich proteoglycan, has been proposed to be the potent tgf-β modulator. in this study, the therapeutic effects of fibromodulin in the kidneys of streptozotocin (stz)-induced diabetic rats were investigated. methods: diabetic rats rec...

esophagus cancer is the eighth most common cancer worldwide and particularly high in an area extending from the southern border of the caspian sea in iran across central asia to china. since information about this mysterious disease is poor, proteomics may be solving this enigma. altering gene expression in cancer cell is a remarkable indicator can be detected by proteomics techniques and bioin...

endometriosis is a painful reproductive disease afflicting about up to 20% of women. it is one of the most frequent benign gynaecological diseases, however, little is known about the pathological of endometriosis. over the past decade, high-throughput proteomics technologies have evolved considerably and have become increasingly more commonly applied to the investigation of female reproductive ...

The general theme of the meeting was the application of mass spectrometry to pharmaceutical and biotechnological research. The majority of the oral presentations and posters were concerned with the development and application of all mass spectrometric techniques related to proteomics.

High-throughput techniques are currently some of the most promising methods to study molecular biology, with the potential to improve medicine and enable new biological applications. In proteomics, the large scale study of proteins, the leading method is mass spectrometry. At present researchers can routinely identify and quantify thousands of proteins in a single experiment with the technique ...