Genetic analysis of the location of a mini-Tn5 promoted insertion of the LB400 bph operon in the rhizosphere coloniser Pseudomonas fluorescens F113rifPCB, allowed the development of a specific PCR detection system based on the unique DNA sequence at this insertion site. Real time PCR using both SYBR green chemistry and Fluorescence Resonance Energy Transfer probes allowed the precise identifica...

Damping-off diseases incited by different species of Pythium are a persistent problem worldwide, often resulting in reduced yields and occasionally resulting in major crop damage. There have been increasing restrictions on the use of chemical fungicides, and the development of disease-suppressive biocontrol agents has become a major goal of horticulture crops. An experiment was conducted with t...

Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered ...

The utility of peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Acinetobacter spp. and Pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of ATCC strains and clinical isolates with select gram-negative rods. After testing 60 clinical isolates, PNA FISH had a sensitivity and specificity of 100% and 100%, respectively, for Ac...

A pot experiment was carried out at screen house, Faculty of Agriculture University Maiduguri to compare the effect NPK, bio-fertilizers and manures applications on growth nutrient uptake by maize. The consisted 9 treatments combined application NPK half full recommended rates for maize, Azotobacter chroococcum, Bacillus megaterium Pseudomonas fluorescence biofertilizers cow dung poultry replic...

Bacteriophages (phages) are viruses that attack bacteria, causing them to multiply. This requires phage orientation with respect the bacterial receptor, a necessary condition for attachment. Since phages not motile, they rely on their Brownian motion and, specifically, its rotational components reorient. We focus specifically Pf1 (the bacteriophage called pseudomonas Pf1), about which much has ...

Bacteria secrete a variety of compounds important for nutrient scavenging, competition mediation and infection establishment. While there is a general consensus that secreted compounds can be shared and therefore have social consequences for the bacterial collective, we know little about the physical limits of such bacterial social interactions. Here, we address this issue by studying the shari...

Prokaryotes represent one-half of the living biomass on Earth, with the vast majority remaining elusive to culture and study within the laboratory. As a result, we lack a basic understanding of the functions that many species perform in the natural world. To address this issue, we developed complementary population and single-cell stable isotope ((13)C)-linked analyses to determine microbial id...