Cytochrome P4501IEI is responsible for the activation of carcinogenic N-nitrosamines, benzene, urethane, and other low-molecularweight compounds. Restriction fragment length polymorphisms (Pstl and RsaI restriction enzymes) have been identified in the cytochrome P4501IE1 transcription regulatory region that may affect expression. This study describes the PstI and RsaI polymorphisms in different...

background: indigenous chickens could serve as precious genetic resources that should be considered in conservation and breeding programs. the major histocompatibility complex (mhc) has a strong association to disease resistance/susceptibility, production and reproduction traits in chicken. therefore, identifying its polymorphism in populations under selective breeding could be used for selecti...

There is a possible association between acute leukemia (AL) and polymorphisms in genes coding for cytochrome P4502E1 (CYP2E1) and myeloperoxidase (MPO), the enzymes both involved in the metabolism and bioactivation of xenobiotic compounds, including benzene especially. Previous studies reported conflicting results. Therefore, a meta-analysis of available molecular epidemiologic studies was perf...

The gene coding for xylulokinase has been isolated from the yeast Pachysolen tannophilus by complementation of Escherichia coli xylulokinase (xylB) mutants. Through subcloning, the gene has been localized at one end of a 3.2-kilobase EcoRI-PstI fragment. Expression of the cloned gene was insensitive to glucose inhibition. Furthermore, the cloned gene did not cross-hybridize with E. coli and Sac...

SOURCE/DESCRIPTION; A 2.0 kb Mspl fragment from cosmid YNH24 isolated by HBV-2 oligonucleotide (GGAGTTGGGGGAGGAG) (1) was subcloned into the AccI site of pUC18. POLYMORPHISM: Mspl identifies a >30 allele VNTR polymorphism with bands between 1.0-5.0 kb. Taql, Bglll, Pvull, PstI and BamHI also detect the same polymorphism.

SOURCE AND DESCRIPTION OF CLONE: 0.6 kb anonymous lymphocyte cDNA cloned into the PstI site of pUR291. POLYMORPHISM: Bglll identifies a two allele polymorphism with a band at either 5.2 kb (A 1) or 5.0 kb (A 2) and invariant bands at approximately

Plasmid pUA466, a 45-kilobase transmissible tetracycline resistance plasmid from Campylobacter jejuni was mapped with AvaI, AvaII, BclI, HincII, PstI, XhoI, and XbaI. The resistance determinant was cloned and expressed in Escherichia coli and was homologous with a class M determinant from Streptococcus spp.