Southern rice black-streaked dwarf virus (SRBSDV) is transmitted efficiently only by white-backed planthopper (WBPH, Sogatella furcifera) in a persistent propagative manner. Here we used a yeast two-hybrid system to investigate the interactions between the SRBSDV- P10 and the cDNA library of WBPH. Of 130 proteins identified as putative interactors, 28 were further tested in a retransformation a...

Aim To explore the expression and clinical significance of long noncoding RNA (lncRNA) gastric cancer-associated transcript 3 (GACAT3) in human colorectal cancer (CRC). Methods Expression of GACAT3 in CRC tissues and cell lines was measured using quantitative real-time PCR. CCK-8 and colony formation assays were used to assess the effect of GACAT3 on CRC cell line proliferation. Transwell inv...

BACKGROUND Linc00152 has been identified highly associated with the tumorigenesis and development of gastric cancer, however, the detailed mechanism of Linc00152 involved still remains unclear. METHODS RT-PCR and western blot were used to detect the expression of Linc00152 and EGFR. The CCK8 and EDU assay was employed to measure cell proliferation while xenotransplantation technology was appl...

In the latent infection of Kaposi's sarcoma-associated herpesvirus (KSHV), its 160-kb circularized episomal DNA is replicated and maintained in the host nucleus. KSHV latency-associated nuclear antigen (LANA) is a key factor for maintaining viral latency. LANA binds to the terminal repeat (TR) DNA of the viral genome, leading to its localization to specific dot structures in the nucleus. In suc...

Background: MTT assay is a colorimetric test to evaluate cell metabolic activity of living cells via reduction the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide insoluble formazan crystals by mitochondrial activities. The tetrazolium dye thought occur NADPH-dependent oxidoreductase enzymes in cytosol. used measure cytotoxicity or cytostatic generally plant and chemical compounds ...

cancer is one of the main cause of mortality in the world which appears by the effect of enviromental physico-chemical mutagen and carcinogen agents. the identification of new cytotoxic drug with low sid effects on immune system has developed as important area in new studies of immunopharmacology. curcumin is a natural polyphenol with anti-oxidative, anti-inflammatory and anti-cancer properties...

rifampin, an antibiotic widely used for the treatment of mycobacterial infections, produceshepatic, renal and bone marrow toxicity in human and animals. in this study, the protectiveeffects of vitamin c and n-acetylcysteine (nac) on the toxicity of rifampin on hepg2 cellswere investigated.human hepatoma cells (hepg2) were cultured in 96-well m of rifampin in the presence ofmicroplate and expose...

organotypic spinal cord slices from neonatal mammals could be a powerful model for evaluation of cell survival but also cell death mechanisms. the aim of this study was to establish an in vitro model for investigating cell survival and mechanism involved in cell death in neonatal spinal cord slices. the spinal cord was sliced and incubated into culture medium. the mtt assay was carried out to a...