Time-resolved adsorption behavior of a human immunoglobin G (hIgG) protein on a hydrophobized gold surface is investigated using multitechniques: quartz crystal microbalance/dissipation (QCM-D) technique; combined surface plasmon resonance (SPR) and Love mode surface acoustic wave (SAW) technique; combined QCM-D and atomic force microscopy (AFM) technique. The adsorbed hIgG forms interfacial st...

In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies for the immunodiagnostics of the visceral leishmaniasis (VL). This chimeric protein was formed by the union in a same artificial coding DNA of ...

There is a consistent demand for new biosensors for the detection of protein targets, and a systematic method for the rapid development of new sensors is needed. Here we present a platform where short unstructured peptides that bind to a desired target are selected using M13 phage display. The selected peptides are then chemically synthesized and immobilized on gold, allowing for detection of t...

This work develops a label-free gliadin immunosensor that is based on changes in the frequency of a quartz crystal microbalance (QCM) chip. A higher sensitivity was obtained by applying 25 nm gold nanoparticles (AuNPs) to the surface of a bare QCM electrode. Subsequently, chicken anti-gliadin antibodies (IgY) were immobilized directly on the AuNP-modified surface by cross-linking amine groups i...

Quartz crystal microbalance (QCM) is a high sensitive chemical sensor which has found widespread spectrum of applications. There are several mechanisms that are related to fluctuation phenomena. Since the aim of our research is oriented to study the sensitivity and influence of different kind of noises on sensor resolution, we modified an existing method to measure the small frequency fluctuati...

Molecularly imprinted polymers (MIPs) as artificial antibodies have received considerable scientific attention in the past years in the field of (bio)sensors since they have unique features that distinguish them from natural antibodies such as robustness, multiple binding sites, low cost, facile preparation and high stability under extreme operation conditions (higher pH and temperature values,...

The research presented in this thesis addresses the problem of updating an analytical model using a parametric Reference Basis approach. In this method, some parameters are assumed to be accurate (e.g. natural frequencies, mode shapes and mass matrix), while others are adjusted so that the eigenvalue equation is satisfied. Updating is done with the use of principal submatrices, and the method s...

Surfaces with antifouling properties are critical for optimizing biosensors to improve the selectivity and specificity of analyte detection in complex biological samples. This work describes four-step synthesis 3-dithiothreitol propanoic acid (DTTCOOH), a new thiol linker that (a) significantly reduces fouling raw human serum samples (b) binds amino receptors via its terminal carboxylic group. ...

Abstract Viscosity measurement has recently captured considerable attention due to its wide range of applications in fields such as pharmacy, food industry, cosmetic and biomedical diagnostics. Acoustic wave sensors quartz crystal microbalance (QCM) are well-known mass that also show their capability measuring liquid viscosity. However, the challenges for QCM-based viscosity devices lie low sen...

The aim of this study was to investigate the oxidation kinetics copper at low temperatures (60 °C 100 °C) in air by isothermal thermogravimetric analysis (TGA) and quartz crystal microbalance (QCM). weight change tests showed periodic increase decrease. In mass sample increased until gradually slowed down finally started decrease due cracking spalling oxide formed on surface. QCM using electrod...