Adult neurogenic niches harbor quiescent neural stem cells; however, their in vivo identity has been elusive. Here, we prospectively isolate GFAP(+)CD133(+) (quiescent neural stem cells [qNSCs]) and GFAP(+)CD133(+)EGFR(+) (activated neural stem cells [aNSCs]) from the adult ventricular-subventricular zone. aNSCs are rapidly cycling, highly neurogenic in vivo, and enriched in colony-forming cell...

A Monte Carlo simulation of multiple trabecular bone cavities in adult bone was developed and the absorbed radiation dose factors evaluated for 3H and 14C. The model was developed to assess the dose from radionuclide uptake in quiescent bone, but also the effects of temporal changes in bone turnover by incorporating bone-modelling units (BMU). Absorbed dose fractions were calculated for target ...

This paper presents a practical method and associated tool for verifying deadlock freedom properties in guarded command systems. Such properties are expressed in CTL as AGEF q where q is a set of quiescent states. We require the user to provide transitions of the system that are “helpful” in reaching quiescent states. The distributed search constructs a path consisting of helpful transitions fr...

Stem cells, one of the progenitors of cancer, exist predominately in a quiescent state. Thus, understanding the mechanisms of DNA repair and mutagenesis in such arrested cells may help unravel the complex process of tumorigenesis. Two major nucleotide excision repair (NER) pathways are known to remove bulky physical or chemical lesions from DNA. Transcription-coupled repair (TCR) acts solely on...

Antineoplastic intercalating agents such as 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA) stabilize a cleavable com plex between topoisomerase II and DNA. The production of proteinassociated DNA cleavage in whole cells exposed to m-AMSA is thought to represent the cellular correlate of this topoisomerase II-mediated reaction. Protein-associated DNA cleavage can be quantified in mam ma...

The mechanisms that bring galaxies to strongly reduce their star formation activity (star-formation quenching) is still poorly understood. To better study galaxy evolution, we propose a classification based on the maps of ionised hydrogen distribution, traced by kpc-resolved, equivalent width H$\alpha$ maps, and nuclear using information from BPT diagnostic diagrams. Using these tools, group sa...

The quiescent state plays an important role in tumor recurrence because it protects cancer cells from chemotherapy. Previously, we optimized tumorsphere cultures for in vitro screening methods for targeting quiescent cell population since the majority of cells in tumorspheres are quiescent. In this study, we analyzed efficacies of current chemotherapeutics in tumorsphere assays to seek better s...

Regeneration of damaged tissues typically requires a population of active stem cells. How damaged tissue is regenerated in quiescent tissues lacking a stem cell population is less well understood. We used a genetic screen in the developing Drosophila melanogaster eye to investigate the mechanisms that trigger quiescent cells to re-enter the cell cycle and proliferate in response to tissue damag...