The prime purpose of this project was to develop and evaluate a method to digitally alter the dynamic range of digital radiographic images. This digital manipulation of the dynamic range can achieve alteration of the displayed contrast and brightness of the digital radiographic image, based on the underlying anatomy within the image. So-called anatomically shaped radiographic contrast-enhanceme...

A simple technique of dynamic radionuclide venographyusing a Searle LFOV gamma camera and an associated whole-body scanning couch-was investigated to demonstrate venous flow from the foot to the inferior vena cava on a single image. Results of this method were correlated with radiographic contrast venography in prospective studies in 84 patients. Correlation between radionuclide and contrast ve...

Renal dysfunction associated with contrast media (CM) administration is generally attributed to reduced renal blood flow. Studies, however, also suggest direct tubular effects of CM, whose mechanisms remain unclear. This study was conducted to assess the chemotoxic effects of iopamidol, a prototypic CM, on a porcine proximal tubule (PT) cell line, LLC-PK(1) cells. Results indicate that iopamido...

Laboratory-based phase-contrast microscopy is a practical and versatile technique for X-ray imaging and tomography of a wide variety of samples. Using phase-retrieval techniques we can obtain quantitative information about the sample as well as making images easier to interpret. We have demonstrated resolution of <60nm in XuM imaging by coupling phase-retrieval with deconvolution methods for re...

For detailed biomedical observations using the optimum phase-contrast x-ray imaging, quantitative comparisons of imaging performances of two major imaging methods--x-ray interferometric imaging (XII) and diffraction enhanced imaging (DEI)--were performed. Density sensitivity and spatial resolution of each imaging method were evaluated using phantom tomograms obtained by each method with the sam...

Phase contrast in X-ray imaging provides lower radiation dose, and dramatically higher contrast at multi-keV photon energies when compared with absorption contrast. We describe here the use of a segmented detector in a scanning transmission X-ray microscope to collect partially coherent bright field images. We have adapted a Fourier filter reconstruction technique developed by McCallum, Landaue...

Novel radiography approaches based on the wave nature of x-rays when propagating through matter have a great potential for improved future x-ray diagnostics in the clinics. Here, we present a significant milestone in this imaging method: in-vivo multi-contrast x-ray imaging of a mouse using a compact scanner. Of particular interest is the enhanced contrast in regions related to the respiratory ...