We describe the design and synthesis of a new Tc-99m labeled bioconjugate for imaging activated complement, based on Short Consensus Repeats 1 and 2 of Complement Receptor 2 (CR2), the binding domain for C3d. To avoid non specific modification of CR2 and the potential for modifying lysine residues critical to the CR2/C3d contact surface, we engineered a new protein, recombinant CR2 (rCR2), to i...

BACKGROUND Noscapine, an alkaloid from Papaver somniferum, widely used as an antitussive, is being clinically studied in the treatment of polycystic ovary syndrome (PCOS) and a few other cancers primarily because of its anti-angiogenesis properties. With the advent of diverse application of noscapine, we sought to determine whether the radiolabeling method can be useful in studying uptake and k...

A series of water-soluble chiral cyclen-based chelators with chemical handles for selective targeting have been synthesized (cyclen = 1,4,7,10-Tetraazacyclododecane). Optical studies, relaxivity measurements, and competitive titrations were performed to show the versatility these chelators. The complexations L3, L4, L5 Lu3+, Y3+, Sc3+, Cu2+ successfully demonstrated in around 90% 100% yields. E...

( B. D etermination of Cellular Heme Levels by Radiolabeling of Yeast Cells In Vivo C. D etermination of Fe/S Cluster Formation in Isolated Mitochondria In Vitro D. A nalysis of Fe/S Cluster Formation In Vitro Using Recombinant Ferredoxins III. D etermination of Mitochondrial Iron

Biosynthesis of ochratoxin A by Aspergillus ochraceus Wilh. was investigated by radiolabeling experiments in which phenylalanine-1-(14)C and sodium acetate-2-(14)C were supplied to the fungus in sucrose-yeast extract medium. Results showed that phenylalanine was incorporated unaltered into the phenylalanine moiety of ochratoxin A, whereas the isocoumarin moiety of ochratoxin A was mostly derive...

Introduction: Venoms are a mixture of proteins and peptides which can be used for fighting cancers. They can affect the exposed fibers and the muscles directly or through motor nerves, causing neuromuscular intoxication. ICD-85 is a combination of three poly-peptides derived from the venoms of an Iranian brown snake and a yellow scorpion. Materials and Methods...