Due to its cost effectiveness, next-generation sequencing of pools of individuals (Pool-Seq) is becoming a popular strategy for characterizing variation in population samples. Because Pool-Seq provides genome-wide SNP frequency data, it is possible to use them for demographic inference and/or the identification of selective sweeps. Here, we introduce a statistical method that is designed to det...

The phylogenetic structure of ecological communities can shed light on assembly processes, but the focus of phylogenetic structure research thus far has been on mature ecosystems. Here, I present the first investigation of phylogenetic community structure during succession. In a replicated chronosequence of 30 sites in northeastern Costa Rica, I found strong phylogenetic overdispersion at multi...

Weiyuan gas field, located in the Sichuan basin of south China, is a large marine gas field with the oldest reservoir (the Sinian sequences) in south China. The hydrocarbon origin of the gas field has long been debated by petroleum geologists. Recently, it was recognized that a paleo-oil pool maybe the significant contributor to the gas field. Consequently, when the paleo-oil pool had been crac...

We have constructed a plasmid, YEpl3, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2/~ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine per...

In a search for genes responsible for the accumulation of antimonite in Escherichia coli, TnphoA was used to create a pool of random insertional mutants, from which one antimonite-resistant mutant was isolated. Sequence analysis showed that the TnphoA insertion was located in the glpF gene, coding for the glycerol facilitator GlpF. The mutant was shown to be defective in polyol transport by Glp...

We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments wer...

Status of this Memo This memo provides information for the Internet community. It does not specify an Internet standard of any kind. Distribution of this memo is unlimited. Abstract This document describes a method for making random selections in such a way that the unbiased nature of the choice is publicly verifiable. As an example, the selection of the voting members of the IETF Nominations C...

Methods We searched Medline, EMBASE, reference lists and forward citations of relevant articles from 1980 to the 19th of April 2013. We included articles which developed a multivariate statistical model to predict recurrent stroke and MI after ischaemic stoke. We extracted data in duplicate using a validated data extraction form. We assessed model quality using pre-defined criteria and aimed to...

The quantum-mechanical description of coherent light, as produced by lasers, gives rise to an intrinsic noise, known as quantum noise, optical noise or shot noise. Several protocols have been proposed to exploit this physical phenomenon to obtain secure data encryption and key distribution. Here we focus on the cryptographic aspects of Barbosa’s protocol[1] and propose an improvement, which is ...

A new circuit combining a dedicated memory controller, standard random-access memory organized as a pool or buffers, and an associative memory designed for executing fast string operations is presented. The three components are designed to work together as a hierarchical in-memory device, carrying out basic string search, insertion, and deletion operations in a single clock cycle, making it ide...