neuronal and astroglial cells were prepared from whole brain of three month and 30-month- old rats for study of alterations in the nuclear poly adp-ribosylation and dna breaks with age. the relative purity of the cell preparations was confirmed by the determination of the neurofilament (low molecular weight) and glutamine synthetase content of the cells using elisa. an increase (75%) in the pol...

Background. Metabolic syndrome (MS) is a complex of disorders characterized by abdominal obesity, insulin resistance and glucose tolerance, arterial hypertension, all types metabolic disorders. Taking into account the wide range symptoms accompanying MS, use preparations with pleiotropic effects on processes in body could be promising for its treatment.
 Objective. The aim this study compa...

The DNA labile sites induced by two nitrosoureas, nimustine (ACNU) and ramustine (MCNU) synthesised in Japan, have been examined in highly reiterated DNA sequences of rat glioma cells. Reiterated fragments of 167 and 203 base pairs (bp), obtained after Hind III and Hae III restriction endonuclease digestion of rat glioma cells DNA, were used as target DNA sequences to determine the labile sites...

Three kinds of apurinic/apyrimidinic (AP) DNA endonuclease, APcI, APcII, APcIII, were purified from rat liver chromatin through 1M KCl extraction, DEAE-trisacryl ion exchange chromatography. Sephadex G-150 gel filtration and AP DNA cellulose affinity chromatography. Activities of the purified APcI, APcII and APcIII were 62.5, 83.3 and 52.0 EU/mg of protein, respectively. Molecular weights of AP...

Two nonmitochondrial forms of DNA polymerase are demonstrated in regenerating rat liver and Morris hepatomas 7777, 7800, and 7794A. The enzymes have been partially purified from subcellular fractions of these tissues. The purification procedure involves fractionation with ammonium sulfate and chromatography on diethylaminoethyl cellulose and on phosphocellulose. The enzymes have different physi...

background: the aim of this study was optimization of the polyfect gene delivery method of pcdna3.1 expression vector transfected with the mouse pdx-1 gene in three different kinds of mesenchymal stem cells and hepa cells as well as comparison of transfection efficiency leading to expression of the mentioned gene in the cell types used. materials and methods: rat bone marrow-derived mesenchymal...

Long and short repetitive sequences of rat DNA can be isolated and characterized. Long [greater than 1.5 kilobases (kb)] sequences can be separated from short (0.2-0.4 kb) sequences by exclusion chromatography after renaturation of 4-kb DNA fragments to a repetitive Cot and digestion with the single-strand-specific S1 nuclease. (Cot is the initial concentration of DNA in mol of nucleotides/lite...

Objective(s):The aim of this study was to evaluate the oxidative status and DNA integrity in testes of wistar rat offspring exposed to omega-9 monounsaturated (MUFA) at different times of late organogenesis. Materials and Methods:Sixty female rats were divided into six groups of 10 animals. The first group served as control and received the drug vehicle, olive oil (1 ml/kg/day). The second, thi...

Background: Co-supplementation of unsaturated fatty acids (FAs) with saturated FAs may decrease the adverse effects of saturated FA-induced lipotoxicity. The objective of the present study was to evaluate the effect of palmitoleic acid (unsaturated fatty acid) on palmitic acid (saturated fatty acid) induced lipotoxicity criteria in the primary culture of adult rat cardiomyocytes. Methods: Ce...