background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

conclusions according to this research and prevalence of hhv-6 in children, evaluation of csf (detecting the hhv-6 dna by pcr) is recommended in patients younger than 13 months with possible encephalitis. results from a total of 114 patients, hhv-6 was detected in 10 (8.8%), 90% of which were boys with mean age 7.7 months and median of 7.5 months. no significant differences were found in clinic...

Cupper Oxide structures with a variety of novel morphologies are synthesized using cupper foil as substrate via a solution route. The structure, morphology and phase of the as-synthesized nanostructures are analyzed by various techniques. SEM images show gradual development of hierarchical structures of copper oxide with different morphology. In order to study the effect of reaction time and te...

Time-sensitive network experiments are difficult. There are major challenges in generating realistic traffic with highly accurate packet arrivals, and monitoring various system performance metrics which require high precision in timing is not trivial. Parts of these problems arise from the fact that generic network software and hardware components do not provide high-precision timing guarantees...

This paper describes various time-domain methods useful for analyzing the experimental data obtained from a circular cylinder force in terms of both wave and current for estimation of the drag and inertia coefficients applicable to the Morison’s equation. An additional approach, weighted least squares method is also introduced. A set of data obtained from experiments on heavily roughened circul...

Alkaline hydrolysis of ethyl acetate is essentially an irreversible and second order reaction. Industrial importance of the reaction product, sodium acetate, necessitate for process improvement in terms of maximum conversion and economical usage of raw materials. Statistical design of experiments was utilized to enhance conversion in both batch and plug flow reactors.A full two level factor...