In this work, we have used novel mAbs against two proteins of the endoplasmic reticulum and outer nuclear membrane, termed NEP-B78 and p65, in addition to a polyclonal antibody against the inner nuclear membrane protein LBR (lamin B receptor), to study the order and dynamics of NE reassembly in the Xenopus cell-free system. Using these reagents, we demonstrate differences in the timing of recru...

We propose a graph-based optimization framework for automatic 2D image fragment reas-sembly. First, we compute the potential matching between each pair of the image fragments based on their geometry and color. After that, a novel multi-piece matching algorithm is proposed to reassemble the overall image fragments. Finally, the reassembly result is refined by applying the graph optimization algo...

Optical switching will enable core Internet packet switching to scale with future transmission rate increases. Currently proposed optical ATM switches do not allow packet reassembly, which is necessary for packet level forwarding. This results in the requirement to create end to end ATM virtual connections for flows even if they contain only one packet. In electronically switched networks MPOA ...

The mammalian Golgi complex consists of stacks of cisternae linked laterally into a continuous perinuclear ribbon structure. Protein kinase A is stably associated with the Golgi complex during interphase. To analyze its role in Golgi structural maintenance cells were depleted of protein kinase A regulatory subunits using small interfering RNAs. Under these conditions, the catalytic subunits red...

The role of the Nuclear Factor κB (NF-κB) transcription factor family in T cell function has been well described. The c-Rel family member is of particular importance in initiating T cell responses to antigen and regulating activation of inflammatory cytokine genes, including the Interleukin-2 (IL-2) and Granulocyte macrophage colony stimulating factor (GM-CSF) genes. c-Rel is required for chrom...

Reassembly of enzymes from peptide fragments has been used as a strategy for understanding the evolution, folding, and role of individual subdomains in catalysis and regulation of activity. We demonstrate an oligomerization-assisted enzyme reassembly strategy whereby fragments are covalently linked to independently folding and interacting domains whose interactions serve to promote efficient re...

Recent measurements of packet networks have shown that packet traffic exhibits long-range-dependent property. Studies have also discovered that this property is not adequately captured by the conventional Markov traffic models and often yields queueing behavior such as heavy-tailed or subexponential queue length distribution. These findings have led to the implication that long-range-dependent ...

To match the signatures of malicious traffic across packet boundaries, network-intrusion detection (and prevention) systems (NIDS) typically perform pattern matching after flow reassembly or packet reordering. However, this may lead to the need for large packet buffers, making detection vulnerable to denial-of-service (DoS) attacks, whereby attackers exhaust the buffer capacity by sending long ...