The glycosylation of recombinant β-glucocerebrosidase, and in particular the exposure of mannose residues, has been shown to be a key factor in the success of ERT (enzyme replacement therapy) for the treatment of GD (Gaucher disease). Macrophages, the target cells in GD, internalize β-glucocerebrosidase through MRs (mannose receptors). Three enzymes are commercially available for the treatment ...

Taliglucerase alfa is an intravenous enzyme replacement therapy approved for treatment of type 1 Gaucher disease (GD), and is the first available plant cell-expressed recombinant therapeutic protein. Herein, we report long-term safety and efficacy results of taliglucerase alfa in treatment-naïve adult patients with GD. Patients were randomized to receive taliglucerase alfa 30 or 60 U/kg every o...

The genome of feline herpesvirus type 1 (FHV-1), the major cause of viral upper respiratory disease in cats, contains several genes encoding homologues of herpes simplex virus type 1 (HSV-1) glycoproteins. Restriction mapping studies have indicated that the group D genome of FHV-1 contains a unique short region that is 9.0 kb long. The nucleotide sequence of a 6.2 kb portion of this region was ...

Fragments of glycoprotein G (gG-2(281-594His)), comprising residues 281 to 594 of herpes simplex virus type 2 (HSV-2), glycoprotein G of HSV-1 (gG-1(t26-189His)), and glycoprotein D of HSV-1 (gD-1(1-313)), were expressed in the baculovirus expression system to develop an assay for the detection of HSV-1 and HSV-2 type-specific antibodies. The expression of the gG-1(t26-189His) and gG-2(281-594H...

Regulated exocytosis involves the Ca(2+)-triggered fusion of secretory vesicles with the plasma membrane, by activation of vesicle membrane Ca(2+)-binding proteins [1]. The Ca(2+)-binding sites of these proteins are likely to lie within 30 nm of the vesicle surface, a domain in which changes in Ca2+ concentration cannot be resolved by conventional fluorescence microscopy. A fluorescent indicato...

OBJECTIVE The ability of recombinant human interferon beta-1a (rh-IFN beta-1a) to suppress multiple sclerosis activity, evaluated from MRI, was assessed across a range of lesions enhancing at different gadolinium-DTPA (Gd) doses and with different sizes. METHODS Every 4 weeks, standard dose (Sd; 0.1 mmol/kg Gd) and triple dose (Td; 0.3 mmol/kgGd) MRI were obtained from 18 patients with relaps...

the mononuclear gd(iii) complex, [gd(l)3(h2o)5] (where l is alizarin yellow r (nac13h8n3o5)), has been prepared in h2o under reflux condition. the gd(iii) complex has been characterized by elemental analysis and spectroscopic methods (uv–vis and ft–ir). the gd2o3 nanoparticles were prepared by the calcination of the gd(iii) complex in air at different temperatures up to 600 °c for 2 h. the calc...

Novel photocatalysts Gd₂FeSbO₇, Gd₂InSbO₇ and Gd₂YSbO₇ were synthesized by the solid state reaction method for the first time. A comparative study about the structural and photocatalytic properties of Gd₂MSbO₇ (M = Fe, In, Y) was reported. The results showed that Gd₂FeSbO₇, Gd₂InSbO₇ and Gd₂YSbO₇ crystallized with the pyrochlore-type structure, cubic crystal system and space group Fd3m. The lat...

The eigenvalues of the isotropic magnetic interaction system extraction from Internal Field Approximation (IFA) q e computed. on the basis of RKKY for the complicated magnetic behaviour of Gd, X system X= (Au , Pt , Al , In ) as well as Gd. From the strength and slope of eigenvalue curves in different ranges of interaction the strength and sharpness of the magnetic transition can be suggest...