one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...

The differentiation of pluripotent stem cells is associated with extensive changes in metabolism, as well as widespread remodeling of the epigenetic landscape. Epigenetic regulation is essential for the modulation of differentiation, being responsible for cell type specific gene expression patterns through the modification of DNA and histones, thereby establishing cell identity. Each cell type ...

Adipose tissue is a complex, highly metabolically active tissue. The expansion of adipose mainly affected by two processes: the number adipocytes and change adipocyte size. Adipocyte differentiation regulated multiple factors. 3T3-L1 preadipocyte line has become most widely studied cell for proliferation, regulation related genes. In our previous study, we found that ANGPTL4 was to through PPAR...

background: culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. methods: in this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. protein profile of recombinant cho (rcho) ce...

ABSTRACT Cellular metabolism has recently emerged as a key regulator of stem cell behavior. Various studies have suggested that metabolic regulatory mechanisms are conserved in different niches, suggesting common level regulation across tissues. Although the balance between glycolysis and oxidative phosphorylation been shown to be distinct cells their differentiated progeny, much less is known ...

the present study was conducted to test the effect of origanum majoranum extract (ome) of leaves on alterations induced in a model of type 2 diabetic rats. adult male wistar rats were fed high fat diet for 3 weeks and injected a single dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetic rats. diabetic rats were given aqueous extract of ome in a dose of 20 mg/kg orally ...

introduction: tuberculosis (tb) remains a deadly infectious disease despite all the efforts to reduce its incidence. spread of multidrug resistant tb has seriously undermined the efforts to control the disease globally. in this study protein expression profile of mdr and sensitive isolates of mtb were analyzed and compared in order to identify proteins, which could be used in prevention, diagno...

autophagy is a cellular process mediating degradation of bulk cytoplasm, long-lived proteins and entire organelles. in this process, double-membrane vesicles, the autophagosomes, wrap around portions of cytosol and transport them to the lysosome for degradation. several molecules participate in autophagosome nucleation and elongation, including various components of the class iii pi3k complex, ...