the objective of this study was to produce fish peptone from tuna (thunnus tonggol) viscera, by alcalase. response surface methodology (rsm) was employed for optimizing the temperature and ph. hydrolysis was done in different tempratures (50-65˚c) , ph (8-8. 5) and selected 13 treatments. samples with higher value of protein were used instead of the standard peptones which applied in commercial...

Runx3, a member of Runt-related transcription factor (Runx) proteins with tumor suppressor effect, is a tissue–restricted and cancer related transcription factor that regulate cell proliferation and growth, as well as differentiation. In the present study, exogenous Run3 was transiently expressed in AGS (human gastric adenocarcinoma), with undetectable Runx3 protein and in A549 (human lung carc...

Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...