The modification of high-performance liquid chromatography parameters leads to a more effective oligonucleotide-A purification process. Using various experimental such as buffer, concentration, and pH, method for optimizing the an on reverse-phase C18 column was created. To purify oligonucleotide-A, High-Performance Liquid Chromatography (HPLC), Ultraviolet-Visible Spectrophotometry (UV-Vis), C...

Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins) and some of its metabolites, by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) after solid-phase extraction (SPE) was reported for the first time. The analysis was carried out using a reverse phas...

Diosmin and hesperidin are natural flavonoid glycosides found in various plant materials, mainly in citrus fruits in different concentrations. Diosmin for pharmaceutical use is obtained mainly semi-synthetically from hesperidin. Hesperidin often accompanies diosmin as a natural impurity in different pharmaceutical formulations; therefore, a simple, fast and precise method for the simultaneous a...

A simple method for the analysis of non-derivatized glycans using a reverse phase column on a liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) instrument. The methodology supports both glycomic and proteomic work flows without the necessity of switching columns.

The authors developed a simple and rapid method for quantitation of 2-phenoxyethanol (2-PE) in DTwP vaccine based on reverse phase high performance liquid chromatography (HPLC). The method was simple and reproducible. The sensitivity of the assay was confirmed by spiking known amounts of 2-phenoxyethanol to the vaccine sample.

A reverse phase high-pressure liquid chromatography system for rapid separation of various retinoids (vitamin A and its analogs) with little or no degradation is described. This method permits detection of as little as 22 pmol of retinoic acid. The procedure has been applied to the study of retinoic acid metabolism in vitamin A-deficient hamsters.

Abbreviations: ACN: Acetonitrile; AvBD: Avian Beta-Defensin; CAM: Carbamidomethylation; 1,5 DAN: 1,5 Diaminonaphthalene; DHB: 2,5 Dihydroxybenzoicacid; DTT: Dithiothreitol; FITC: Fluorescein Isothiocyanate; IAA: Iodoacetamide; ISD: In Source Decay; MALDI-MS: Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; m/z: Mass/Charge; PI: Propidiumiodide; RP-HPLC: Reverse Phase-High Pressure...

oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture conten...