New simple and sensitive spectrophotometric and fluorimetric methods have been developed for the determination of prazosin hydrochloride (PRZ) in its active pharmaceutical ingredient (API) and drug product. The spectrophotometric method (Method I) is based on formation of a binary complex with rose Bengal (RB) at 572 nm. The absorbance-concentration plot is rectilinear over the range 2.5 25 μg/...

Objective We have previously shown that Rose Bengal (RB) alone, not as a photosensitiser, could induce apoptotic- and non-apoptotic cell death in different melanoma cell lines. To clarify RB-induced toxicity mechanisms, role of caspases and reactive oxygen specious (ROS) were studied in melanoma cells. Material and Methods Human melanoma cell lines, Me 4405 and Sk-Mel-28 were cultured in DM...

Branch retinal vein occlusion induces complex biological processes in the retina that are generated by a multitude of interacting proteins. These proteins and their posttranslational modifications can effectively be studied using modern proteomic techniques. However, no method for studying large-scale protein changes following branch retinal vein occlusion has been available until now. Obtainme...

PURPOSE An oil in water emulsion of 0.01% all-trans-retinoic acid (tretinoin) was prepared and clinically evaluated in dry eye patients. METHODS The ophthalmic emulsion consisted of 10% of arachis oil and 90% of the hydrogel of Carbopol 940. To evaluate retinoic acid emulsion clinically, a placebo-controlled, open-labeled, randomized study was performed with 22 dry-eye patients. Symptoms were...

Rose bengal plate test (RBPT) antigens from Brucella melitensis and Brucella suis S 2 were prepared and compared with RBPT antigen prepared from classical Brucella abortus S 99. A total of 54 sera samples, of which 7 were collected from humans with brucellosis and 47 from infected sheep, were studied by using RBPT and serum agglutination test (SAT). RBPT and SAT results showed that RBPT antigen...

Aim of the study was to evaluate the diagnostic performance of the Rose Bengal score test for Sjøgren's syndrome (SS), and to explore differences between other tests and examinations. All participants were examined, including (but not limited to) unstimulated (UWS) and stimulated (SWS) whole saliva, labial gland biopsy (LGB or focus score), ophthalmologic questionnaire (ocular surface disease i...

We compared the relative value of the tear film breakup time, Schirmer test, lactoferrin immunologic assay and rose bengal staining in 156 patients with varying severity of symptoms characteristic of keratoconjunctivitis sicca and in 39 controls. Statistical analysis showed that in patients with minimal ocular irritation the Schirmer test in combination with the lactoferrin immunologic assay pr...