To study the molecular basis of biotrophic nutrient uptake by plant parasitic rust fungi, the gene (Uf-PMA1) encoding the plasma membrane H(+)-ATPase from Uromyces fabae was isolated. Uf-PMA1 exists probably as a single gene. However, two nearly identical sequences were identified; the similarity apparently is due to two Uf-PMA1 alleles in the dikaryotic hyphae. Multiple Uf-PMA1 transcripts wer...

63 Bean rust is a devastating disease of common bean in the Americas and Africa. The historically 64 important Ur-3 gene confers resistance to many races of the highly variable bean rust pathogen 65 that overcome all known rust resistance genes. Existing molecular markers tagging Ur-3 for use 66 in marker assisted selection produce false results. We described here the fine mapping of Ur-3 67 fo...

Stripe rust caused by Puccinia striiformis f. sp. tritici is most important and devastating disease of wheat worldwide, which affects the grain yields, quality and nutrition. To elucidate, the genetic basis of resistance, a mapping population of recombinant inbred lines was developed from a cross between resistant Cappelle-Desprez and susceptible cultivar PBW343 using single-seed descent. Varie...

Experienced programmers often need to use online resources to pick up new programming languages. However, we lack a comprehensive understanding of which resources programmers find most valuable and utilize most often. In this paper, we study how experienced programmers learn Rust, a systems programming language with comprehensive documentation, extensive example code, an active online community...

Glyphosate is a broad-spectrum herbicide used for the control of weeds in glyphosate-resistant crops. Glyphosate inhibits 5-enolpyruvyl shikimate 3-phosphate synthase, a key enzyme in the synthesis of aromatic amino acids in plants, fungi, and bacteria. Studies with glyphosate-resistant wheat have shown that glyphosate provided both preventive and curative activities against Puccinia striiformi...

The obligate bitrophic fungus Puccinia striiformis f. sp. tritici (Pst) causes stripe (yellow) rust on wheat worldwide. Here, we report a novel fungal strain able to hyperparasitize Pst. The strain was isolated from gray-colored rust pustules, and was identified as Alternaria alternata (Fr.: Fr.) keissler based on a combination of morphological characteristics and multi-locus (ITS, GAPDH, and R...

The introduction of nonnative pathogens is altering the scale, magnitude, and persistence of forest disturbance regimes in the western United States. In the high-altitude whitebark pine (Pinus albicaulis) forests of the Greater Yellowstone Ecosystem (GYE), white pine blister rust (Cronartium ribicola) is an introduced fungal pathogen that is now the principal cause of tree mortality in many loc...

Stripe rust of barley, caused by Puccinia striiformis f. sp. hordei (PSH), occurred mainly in California, Idaho, Oregon, and Washington and caused localized damage to barley crops. Growing cultivars with high-temperature, adult-plant resistance and the cropping system contributed to the low level of stripe rust. Stripe rust samples were collected from the four states and tested under controlled...

The COP9 (constitutive photomorphogenesis 9) signalosome (CSN) is a protein complex involved in the ubiquitin proteasome system and a common host target of diverse pathogens in Arabidopsis. The known derubylation function of the COP9 complex is carried out by subunit 5 encoded by AtCSN5A or AtCSN5B in Arabidopsis. A single CSN5-like gene (designated as TaCSN5) with three homeologues was identif...

Barilli E., Sillero J.C., Prats E., Rubiales D. (2014): Resistance to rusts (Uromyces pisi and U. viciae-fabae) in pea. Czech J. Genet. Plant Breed., 50: 135–143. Pea is the second most important food legume crop in the world. Rust is a pea disease widely distributed, particularly in regions with warm, humid weather. Pea rust can be incited by Uromyces viciae-fabae and by U. pisi. U. viciae-fab...