Wild-type strains of Escherichia coli are unable to utilize aromatic beta-glucosides such as arbutin and salicin because the major genetic system that encodes the functions for their catabolism, the bgl operon, is silent and uninducible. We show that strains that carry an activated bgl operon exhibit a growth advantage over the wild type in stationary phase in the presence of the rpoS819 allele...

The aim of this study was the development and validation of a new method for carbohydrates analyses obtained from woody biomass, as a step of bioethanol production. In this study, we have quantified carbohydrates in wood by gas chromatography (GC) after derivatization. Quantification of carbohydrates was made by liquid-liquid extraction, oximation and silylation and, finally, analysis by gas ch...

Some bacteria of the soil microflora or of the digestive systems of mammals can grow on aryl beta-glucosides as sole carbon sources. The bgl operon of E. coli is the paradigm for such a catabolic pathway. In Azospirillum irakense, the two adjacent genes salAB encode beta-glucosidases which are required for salicin assimilation. In this report, we analyse the sequence of salC, the last gene to b...

Different extracts of Rhodobryum giganteum Par. were screened with mice model of acute myocardial hypoxia induced by isoproterenol and pituitrin, eight compounds, i.e. p-hydroxycinnamic acid, caffeic acid-4-O-β-d-glucopyranoside, salicin, 7,8-dihydroxy coumarin, menthol, allantoin, palmitic acid, palmitamide were isolated and identified from the active fraction, among which p-hydroxycinnamic ac...

Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943(T) (=CCUG 47727(T)) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptami...

temperature was 27°C. On International Streptomyces Project (ISP) media 1, 2, 3, and 5, the strain produced yellow diffusible pigment and the reverse side color of the colonies was yellow. Melanin production was not observed on either tyrosine or peptone iron agar. The following tests were positive: starch hydrolysis; degradation of allantoin, lecithin, pectin, xanthine, and hypoxanthine; resis...