OBJECTIVES The Japanese Veterinary Antimicrobial Resistance Monitoring (JVARM) Program was established in 1999 to examine the susceptibility of bacteria from food-producing animals to antimicrobial agents. This study tested the susceptibility of Salmonella isolates collected during 2001-2002 to 20 antimicrobials. MATERIALS AND METHODS MICs of antimicrobial agents were determined using the NCC...

An Au-on-Au tip sensor is developed for the detection of Salmonella typhimurium (Salmonella), using a new synthetic nucleic acid probe (NAP) as linker immobilization DNA-conjugated Au nanoparticle (AuNP) onto DNA-attached thin layer inside pipette tip. In presence Salmonella, RNase H2 from (STH2) cleaves NAP and freed AuNP can be visually detected by paper strip. This portable biosensor does no...

Corresponding Author: Shakil Ahmed Department of Biotechnology, Flensburg University of Applied Sciences, Kanzleistr.91-93, 24943 Flensburg, Germany Email: [email protected] Abstract: A very small number of bacterial pathogens may have fatal effects on food safety. In spite of having great advancements in bioanalytical methods, most of the accepted detection methods are still cultiva...

a total of 400 bovine diarrhoeic fecal specimens were obtained and conventional microbial culture, immunomagnetic separation and multiplex pcr were simultaneously carried out on samples. for detection of salmonella at genus level, inv-a universal primer was selected. in order to identifiing of salmonella typhimurium, specific primers of rfbj, fljb and flic related to gene sequence of o4, h2:1,2...

In this study, we have developed an efficient method based on single-stranded DNA (ssDNA) aptamers along with silica fluorescence nanoparticles for bacteria Salmonella typhimurium detection. Carboxyl-modified Tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (RuBPY)-doped silica nanoparticles (COOH-FSiNPs) were prepared using reverse microemulsion method, and the streptavidin was conjugated...

Lipopolysaccharide (LPS) is a major virulence factor present in the outer membrane of Salmonella enterica serovar Typhimurium (S. Typhimurium). Outer membrane proteins (OMPs) from Salmonella show high immunogenicity and provide protection against Salmonella infection, and truncated LPS alters the outer membrane composition of the cell wall. In our previous study, we demonstrated that Salmonella...

background: salmonella typhimurium is one of the most important species of salmonella that is intracellular parasite and attacks host mucus membrane. these bacteria can cause gastroenteritis, and their main transmission route is water, poultry, meat, egg, and raw food. the aim of this study was to detect three virulence genes associated with s. typhimurium named inva, stm4497, and flic183 genes...

The persistence of enteric pathogens on plants has been studied extensively, mainly due to the potential hazard of human pathogens such as Salmonella enterica being able to invade and survive in/on plants. Factors involved in the interactions between enteric bacteria and plants have been identified and consequently it was hypothesized that plants may be vectors or alternative hosts for enteric ...

hiv-related immunosuppression significantly increases the risk of acquiring opportunistic infections. this report describes a 69-year-old man, referred to hospital with decreased consciousness and productive cough. this man was a known case of human immunodeficiency virus positive. the sputum of this patient was positive for salmonella typhimurium. pulmonary auscultation signaled brief and scat...

background : the aim of this study was to compare the antimicrobial activity of watermelon juice probioticated using differ­ent strains of lactobacilli against salmonella typhi murium. methods : probioticated watermelon juice was produced using four strains of lactobacilli (lactobacillus casei, l. acidophil­lus, l. fementum and l. plantarum ). the watermelon juice was pasteurized for 30 minutes...