The presence of two major egg polypeptides was demonstrated in the plant-parasitic nematode Heterodera glycines. The polypeptides were present in equal amounts in, and were most abundant in, eggs from yellow females. They were also present in brown females but were not detected in second-stage juveniles (J2). The two major egg polypeptides, MEP-I and MEP-II, accounted for more than 50% of the t...

The effects of nematicides carbofuran (C) and fenamiphos (F) and herbicides metribuzin (M) and trifluralin (T), alone and in combination, on hatching, penetration, development, and reproduction of Meloidogyne incognita race 3 were determined under laboratory conditions. To study hatching, entire egg masses were exposed to nematicides (6 mug/ml), herbicides (0.5 mug/ml), and their combinations o...

Parasitism genes encoding secreted effector proteins of plant-parasitic nematodes play important roles in facilitating parasitism. An annexin-like gene was isolated from the cereal cyst nematode Heterodera avenae (termed Ha-annexin) and had high similarity to annexin 2, which encodes a secreted protein of Globodera pallida. Ha-annexin encodes a predicted 326 amino acid protein containing four c...

Penetration of second-stage juveniles (J2) of Meloidogyne incognita into tomato root explants and in vitro propagated peach plantlet roots were compared. Five inoculum levels were used: 25, 50, 75, 100, and 200 J2 for tomato; and 50, 100, 200, 500, and 1,000J2 for peach. The greatest root penetration into tomato was 30% at the 75 J2 level, but the maximum penetration into peach roots was only 8...

Three populations of the corn cyst nematode Heterodera zeae, one found in the rhizosphere of a fig tree and two infecting corn, were studied using the morphology and morphometry of cysts and second-stage juveniles, and compared with other populations. The intrapopulation and intraspecific variability are discussed. A simple and improved technique to prepare vulval cones for SEM is described. Th...

Globodem roswch!ens!s and G. pall!da harched (l-day-old in poraro roor diffusare) second srage juveniles 02) conrained 29.2 % and 27.2 % lipid (of dry weighr), respectively. Globodera roslOch!ens!s J2 lipid consisred of 72.8 % neutral lipid, 11.3 % free fatty acids and 15.9% non-acetic phospholipid. The neutral lipid fraction comprised 95.5 % triacylglycerides, 1.8 % diacylglycerides, 2.3 % mon...

ture, only a few second-stage juveniles of M. incognita develop and reproduce. Inoculated tomato genotypes that had only a few egg masses on the average when exposed to continuous high soil temperature showed greater resistance under thermoperiodic conditions than tomato entries showing greater numbers of egg masses when exposed to continuous high soil temperature. T h e results suggest that me...

Experiments were established in field microplots to examine the association between Heterodera glycines and the blue form of Fusarium solani in sudden death syndrome of soybean (SDS). Foliar disease symptoms occurred on more plants per plot, appeared 3 to 7 days earlier, and were more severe on plants grown in plots infested with F. solani + H. glycines than on those inoculated with F. solani o...

Occurrence of cyst nematode Heterodera daverti Wouts and Sturhan 1978 (Wouts & 1978) is reported from Australia for the first time. Cysts were recovered soil samples collected a hop farm in Merriang, Victoria. Morphological morphometric characteristics cysts second stage juveniles match those described this species other parts world. Identification was further supported by molecular evidence th...

The knapweed nematode, Subanguina picridis, forms galls on the leaves, stems, and root collar of Russian knapweed, Acroptilon repens. After being revived from a dormant, cryptobiotic state, second-stage juveniles required at least 1 month in a free-living state before becoming infective. Galls were induced on relatively slow-growing host plants that retained their apical meristems at or near th...