The regulated Cl(-) secretory apparatus of T84 cells responds to several pharmacological agents via different second messengers (Ca(2+), cAMP, cGMP). However, information about water movements in T84 cells has not been available. In the absence of osmotic or chemical gradient, we observed a net secretory transepithelial volume flux (J(w) = -0.16 +/- 0.02 microl.min(-1).cm(-2)) in parallel with ...

Most striated duct (SD) cells in the adult mouse parotid gland (PAG) have a few small secretory granules. These granules, however, are usually too small and sparse to be detected using light microscopy. Our serial studies have suggested that these PAG SD cells belong to a group of hormone-responsive granular duct cells, similar to the granular convoluted tubule (GCT) cells found in the submandi...

The 80K (80 x 10(3) Mr) integral membrane protein, first described in the secretory granules of rat basophilic leukaemia (RBL) cells, is also localized to lysosomes in these cells. The protein displays the same distribution in natural killer lymphocytes (RNK-7), wherein it codistributes with cytolysin in secretory granules. In contrast, the protein is absent from the endocrine and exocrine secr...

Rat pancreatic islets have been studied following successive daily administration of dehydroascrobic acid (DHA) and during the recovery phase following 3 daily injections. One injection of DHA produces degranulation of B cells seen in the light microscope as a loss of aldehyde fuchsin positivity. In the electron microscope the B cells appear to have secretory granules accumulated subjacent to t...

The present study was conducted to determine the antidiabetic effect of Momordica charantia on pancreatic islets Langerhans in streptozotocin-induced diabetic male Wistar rats relation distribution insulin immuno-positive beta cells. fed at 2 %, 5 % and 10 standard diet for 12 w. After sacrifice, tissues were obtained histopathological immunohistochemical observations. In addition, monitoring f...

fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. in this study, dot-elisa, one of the simplest and the most sensitive tests in this regard, was evaluated using excretory-secretory antigens of fasciola hepatica to diagnose human fasciolosis three groups consisting of patients infected with fasciolosis (n= 95), patients with o...

A comparative anatomical study of the stems in 32 taxa assigned two related genera; Matricaria (4 taxa) and Tripleurospermum (28 taxa, 30 accessions), was carried out Turkey by using cluster analysis (CA) principal component (PCA) to address generic classification delimitation. All studied have following stem characteristics: one layer epidermal cells with a sparse distribution non-glandular tr...

Cost-effective microbial biosynthesis of 2?-fucosyllactose (2?-FL) allows its application in infant formula. The specific effects 2?-FL on the gastrointestinal immune barrier are still largely unknown. Here, we quantified and compared HMOs isolated from human milk, 2?-FL/lactose (Lac), expression mucus associated genes MUC2, TFF3, RETLB Golgi sulfotransferases, CHST5, GAL3ST5, goblet cells. We ...

Ultrastructure and acid phosphatase activity in Paneth cells, which are thought to play a role in regulation of the intestinal flora, were observed in control and germ-free rats (5 weeks of age, male). Examination of the germ-free rats revealed: secretory granules with coarse and a low-electron dense matrix in the duodenal, jejunal and ileal Paneth cells; numerous and larger lysosomes in ileal ...

Highly purified Clara cells (93 +/- 3%) isolated from the lungs of rabbits were used to produce an antiserum against Clara cell secretory proteins. This antiserum was used to identify and study the biosynthesis and secretion of [35S]methionine-labeled proteins from isolated Clara cells. The antiserum recognized one major secretory protein with apparent molecular weight of 6 kDa and reacted weak...