development of potent new anti-shigella agents for rapid and specific detection and treatment is of great importance. aptamers, nucleic acid oligomers capable of specific binding to a wide range of non-nucleic acid targets, may be of value for this purpose. in the present study, we used a systematic evolution of ligands by exponential enrichment (selex) process to select dna aptamers that b...

Aptamers are small nucleic acid ligands that bind to their targets with specificity and high affinity. They are generated by a combinatorial technology, known as SELEX. This in vitro approach uses iterative cycles of enrichment and amplification to select binders from nucleic acid libraries of high complexity. Here we combine SELEX with the yeast three-hybrid system in order to select for RNA a...

The SELEX experiment (E781) is a 3-stage magnetic spectrometer for a high statistics study of hadroproduction of charm baryons out to large xF using 650 GeV Σ,π and p beams. The main features of the spectrometer are: a high precision silicon vertex system; powerful particle identification provided by TRD and RICH; forward Λs decay spectrometer; and 3-stage lead glass photon detector. Preliminar...

Nucleic acid aptamers are single-stranded oligonucleotides that interact with target molecules with high affinity and specificity in unique three-dimensional structures. Aptamers are generally isolated by a simple selection process called systematic evolution of ligands by exponential enrichment (SELEX) and then can be chemically synthesized and modified. Because of their high affinity and spec...

Increased awareness of the multiple roles RNA molecules has led to realization that, in addition their structural and functional roles, RNAs can be drug targets for small molecular therapy. Amikacin, a member aminoglycoside group antibiotics, binds specific sites bacterial 16S ribosomal (rRNAs) interferes with protein synthesis leading cell death. Here, we used systemic evolution ligands by exp...

SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is a procedure by which a mixture of nucleic acids can be fractionated with the goal of identifying those with specific biochemical activities. One combines the mixture with a specific target molecule and then separates the target-NA complex from the resulting reactions. The target-NA complex is separated from the unbound NA by m...

We have explored a simplified system without purification for systematic evolution of ligands by exponential enrichment (SELEX) against Vibrio alginolyticus to select single-stranded DNA ligands (aptamers) from a random 82-nt library. The DNA content was quantified by agarose gel electrophoresis and follow-up image analyses with the BandScan 5.0 software. Anti-digoxigenin/HRP system was used to...

Fabio Antonio Bovino1, Maria Cristina Larciprete2, Concita Sibilia2 Maurizio Giardina1, G. Váró3 and C. Gergely4 1Quantum Optics Lab Selex-Sistemi Integrati, Genova, Italy 2Department of Basic and Applied Sciences in Engineering, Sapienza University, Rome, 3Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, 4Montpellier University, Charles Coulomb Labora...