The aims of this study were to compare glycerol (G) at customary concentrations and ethylene glycol (EG) as cryoprotectants for stallion semen in a skimmed milk (SM) extender, to test different EG concentrations and to compare the results of manual and computerized analysis with the hypoosmotic swelling (HOS) test. Ejaculates from two stallions were collected over 3 weeks (6 ejaculates per stal...

The Effect of Resveratrol Antioxidant in Nano Liposome and NLC Forms Rooster Semen Extender During Storage at 4˚C

Spermatozoa, especially those of the porcine species, are highly susceptible to in vitro chilling and ageing. Extenders are continuously developed to protect boar spermatozoa from chilling injury. New semen extenders and other modified preservation strategies require sensitive testing for essential sperm functions. The key process on the pathway of fertilization is capacitation. The aim of the ...

Kozdrowski R., A. Dubiel, W. Bielas, M. Dzięcioł: Two Protocols of Cryopreservation of Goat Semen with the Use of Computer-Assisted Semen Analysis System.Acta Vet. Brno 2007, 76: 601-604. The objective of the study was a comparison of two protocols of goat semen cryopreservation with the use of computer-assisted semen analysis system. Twenty ejaculates obtained with electroejaculation method we...

Microorganisms that inhabit the avian cloaca usually contaminate poultry semen which could easily spread throughout an entire flock. This study was conducted to determine presence of microbial contaminants in turkey and evaluate their antibiotic sensitivity. Semen collected from each tom, pooled then divided into two aliquots A B. Aliquot immediately evaluated for sensitivity while aliquot B, e...

Maintaining a successful pig artificial insemination programme depends on a number of factors, including evaluation of semen characteristics. This study compared the efficacy of different extenders on the sperm motility of Kolbroek semen during short term storage at 4 °C and 25 °C. Semen was collected from Kolbroek boars using the gloved hand technique and transported to the laboratory for eval...

The study was designed to assess the effects and optimal range of osmotic pressure of citrate egg yolk extender (CEYE) for cryopreservation of buffalo bull semen. Semen samples of four Nili-Ravi buffalo bulls, kept for artificial insemination (AI) purposes, were collected at weekly interval for five weeks. Five solutions of tri-sodium citrate dihydrate and D (-) fructose of varying osmotic pres...

This study tested different concentrations of egg yolk in canine freezing extender void of glycerol, a commonly used cryoprotectant, by examining the motility and morphology throughout the freezing process: initial (baseline after extender added), post-cool (after three hours at 5°C) and post-thaw (after freezing.) Initial values of pH, osmolarity, motility and morphology were obtained for comp...

Artificial insemination (AI) using chilled ram semen has not been adopted in Uruguay yet, probably due to a lack of information about the use of this technology in the country. This study evaluated the fertility rate obtained in Merino ewes inseminated with ram semen stored at 5 °C in a TRIS-based extender in Uruguayan field conditions. The effect of storage period of semen (Experiment 1) and d...