This paper describes a microfluidic device fabricated in poly(dimethylsiloxane) that was employed to perform amperometric quantifications using on-chip calibration curves and on-chip standard addition methods. This device integrated a network of Au electrodes within a microfluidic structure designed for automatic preparation of a series of solutions containing an electroactive molecule at a con...

AT THE PRESENT time, indicator-dilution curves are one of the most important technics employed in the investigation of the circulation. The earliest of these studies were carried out by the rapid serial collections of blood samples and their subsequent photometric analysis.' This ingenious but cumbersome method was replaced by the continuous withdrawal of blood in front of a photoelectric cell,...

BACKGROUND Limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of Leishmania infection. Nowadays, real-time PCR is being increasingly applied to quantify infectious agents. In the present study, a real-time PCR assay was developed to estimate parasite burdens in lymph nodes of Leishmania major infected BALB/C mice. Enumer...

Attempts to improve current methods of cryopreservation of corneas, whether by conventional freezing and thawing or by vitrification in the absence of ice, will require the use of high concentrations of cryoprotectants. In this study we extend our previous investigation of the tolerance of rabbit corneas to multimolar concentrations of the cryoprotectant dimethyl sulfoxide (Me2SO) added and rem...

Introduction: Mizaj-e-Advia (Temperament of drug) is the central concept for drug action in traditional Unani medicine. Mizaj-e- Advia determined by different methods, them Qiyas (Analogy) and Tajriba (Experimentation) are conventional one. applied to confirm drugs undertaken comparison physico-chemical properties substances with a known comparator drug. Organoleptic characters i.e.colour, tast...

The ability to taste phenylthiocarbamide (PTC), is autosomal trait inherited in a simple Mendelian recessive pattern. frequency of Taster and non-Taster allele varies different populations. purpose the research investigate prevalence, gene genotype taster (T) non (ts Osing population Kemiren-Banyuwangi. PTC serial dilution method was used assess phenotypes. Hardy–Weinberg determine frequencies....

rue, ruta graveolens, is a medicinal plant in traditional medicine that has several therapeutic properties such as anti-carcinogenic agent, antioxidant, anti-inflammatory, antimicrobial, hypotensive, and spasmolytic. this study was carried out to evaluate the antibacterial effects of the etanolic extract of rue against piscine pathogenic bacteria viz. lactococcus garvieae and aeromonas hydrophi...

Dose-response studies are an essential part of the drug discovery process. They are typically carried out on a large number of chemical compounds using serial dilution experimental designs. This paper proposes a method of selecting the key parameters of these designs (maximum dose, dilution factor, number of concentrations and number of replicated observations for each concentration) depending ...