نتایج جستجو برای: seroepidemiology enzyme immunoassays
تعداد نتایج: 245521 فیلتر نتایج به سال:
The application of molecular technologies, such as the expression of viral proteins in baculovirus, has provided a powerful approach to the diagnosis of human calicivirus (HuCV) infections. The baculovirus-expressed HuCV capsid protein self-assembles into virus-like particles, providing excellent reagents for immunologic assays, such as enzyme immunoassays (EIAs). Following the expression of th...
We studied the EMIT (Enzyme Multiplied Immunoassay Technique, Syva) procedures for the assay of phenytoin and phenobarbital in serum, adapting them to the miniature Centrifugal Fast Analyzer. For different concentrations of drug, each rate of reaction decreased continuously with time, tending to converge on a single common value. The rate was most affected by the concentration of drug almost im...
An enzyme-amplified immunoassay for thyrotropin has been evaluated. The lower limit of detection of the assay (mean + 3 SD of the zero standard) was 0.037 milli-int.unit/L and the precision was good, giving a working range of 0.13-24.0 milli-int.units/L. The accuracy of the assay was good, as judged from analytical-recovery experiments, analysis of external quality-assessment samples and compar...
Published information on the major interactive variables of enzymic immunoassays for drugs, whether in urine or serum, is scanty. Using the Syva EMIT-dau Barbiturates assay as an example, we examined the following interactive variables with respect to test response: concentrations of enzyme, antibody, and drug; urine matrix; and time. The results suggest a process for optimizing this type of en...
Heparin interferes with measurement of aminoglycosides in serum by biological, radioenzymatic, and homogeneous enzyme immunoassay techniques, but not with radioimmunoassay. At concentrations greater than or equal to 10(5) and greater than or equal to 3 X 10(6) USP units/L, respectively, it interferes with the radioenzymatic assay by inhibiting the gentamicin 3-acetyltransferase (EC 2.3.1.60) an...
HBs protein is encoded by the pre-S and S genome, and the detection of HBs antigen (HBsAg) in sera is an initial step for the diagnosis of hepatitis B virus (HBV) infection. The “a” determinant region (amino acid residues 124–147) is the main epitope for inducing a protective immune response. Mutations in the “a” determinant region, known as escape mutants, affect antigenicity and hinder attemp...
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