intradermal injection of autologous serum and plasma elicit a cutaneous reactivity in almost 45-60% of patients with chronic idiopathic urticaria (ciu).   this reactivity is associated with the presence of auto antibodies against ige or ige receptors. this study was carried out to compare the cutaneous reactivity of autologous serum and plasma skin tests in a series of patients with ciu for dia...

 This study was aimed to detect anti-Toxoplasma gondii antibodies in cats infected with Rh strain of T. gondii by using an immunoblotting method. For this, cats were experimentally infected using tachyzoites harvested from Vero cell cultures. Tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to PVDF membranes. An immunoblotting was performed using these mem...

: presence of antisperm antibodies (asas) in infertility and their adverse effects on fertility is a matter of controversy. the aims of this study were to determine the percentage of antibody positive sperms and rate of asa positive sera in both fertile and infertile men and women, and to find the association between these antibodies and infertility.   methods: this study consisted of 29 fertil...

background: antiphospholipid antibodies (apl) are a heterogeneous family of anti-bodies associated with thrombosis and other complications. objective: to study the prevalence of apl in patients with thrombosis at aleppo university hospitals, syria. methods: one hundred and fifty-seven patients with venous and arterial thrombosis and 63 healthy controls were studied. anticardiolipin antibodies (...

Abstract Background and Aims Primary membranous nephropathy (PMN), a common form of the nephrotic syndrome, is an antibody-mediated autoimmune glomerular disease. Autoimmunity driven by circulating autoantibodies that bind to one or more antigens on surface podocytes. PLA2R antigen most prevalent among these antigens. Anti-PLA2R antibodies are measured in serum detected within biopsies primary ...

Abstract Objective This prospective controlled observational cohort pilot study investigated a potential blood-based biomarker [α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)] and relationship to neurocognitive testing in collegiate athletes. Methods A multisport sample of 60 athletes (53% female; 44 contact, 16 non-contact controls) completed ImPACT provided blood sample...

Introduction. One of the widely used specific anti-HER 2 (human epidermal growth factor receptor 2) MAb drugs is trastuzumab. Trastuzumab highly effective for malignant HER hyperexpression reduction, which results in oncogenicity decrease. As any other biotherapeutics trastuzumab can cause immunological adverse reactions, e.g. immunogenicity or anti-drug antibodies (ADAs) production. Aim. The a...

BACKGROUND Many studies have focused on the challenges of small molecule uptake across the blood-brain barrier, whereas few in-depth studies have assessed the challenges with the uptake of antibodies into the central nervous system (CNS). In drug development, cerebrospinal fluid (CSF) sampling is routinely used as a surrogate for assessing CNS drug exposure and biomarker levels. In this report,...

Background: The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Objectives: Development of antibodies against extra cellular domain of influenza M2 (M2e) protein in egg yolk of laying hens. Methods: A Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e ...

Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration anti-spike protein (SP) SARS-CoV SP. The enhanced specificity the to was demonstrated an immunoassay after steps. binding constants (...