We describe a double-antibody enzyme immunoassay for free thyroxin (FT4) in serum with use of beta-D-galactosidase conjugated to thyroxin. The method is uninfluenced by thyroxin-binding globulin or albumin. Values for FT4 so determined correlated well with those determined by radioimmunoassay (r = 0.98) and equilibrium dialysis (r = 0.89). The mean variability (CV) within and between assays was...

1. Three forms of iatrogenic serum enzyme al terat ion are presented, each entailing a different mechanism of action. 2. Recent modifications in creatinine phosphokinase (CPK) assay systems have great ly increased the sensit ivity of this measurement . This increase in sensit ivity has been accompanied by a relative decrease in clinical specificity. In pat ients receiving intramuscular injectio...

Since this symposium is concerned with enzymes in medicine, I shall consider only the work that has been done in human malnutrition. It would be impossible to deal with the very large literature on enzyme changes in animals under different dietary conditions. I shall also confine myself to work which is related to protein and calorie deficiency because this is the field with which I am familiar...

Serial studies of the serum levels of trypsin inhibitor have been performed on monkeys following injection of turpentine-falba, total body irradiation, thermal injury, splenectomy, administration of cortisone acetate and egg albumin. Following the injection of turpentine-falba mixture there was a prompt elevation in the trypsin inhibitor power of the serum. A similar response accompanied the ad...

serum cortisol level was measured by an enzyme immunoassay (eia) using the enzyme penicillinase as a label without prior extraction and purification. polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-cmo) conjugated to bovine serum albumin (bsa). this antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticosteron...

DTIRING THE PAST 12 years or so we have all been performing large numbers of enzyme tests and the present occasion seems appropriate for an attempt to assess the status of enzyme tests in diagnosis. On occasion most of US have obtained results which did not quite fit the clinical diagnosis and in some cases an explanation for the anomalous results can be postulated. In others the puzzle remains...

laboratory diagnosis of acute measles is usually achieved by serology assays for measle-specific igm antibody. for comparison of measle-specific igm antibody in saliva and serum, 95 paired blood and saliva samples were collected 1-14 days after the onset of rash. the specimens were tested for specific igm antibody by an igm antibody-capture enzyme immunoassay (eia). measles igm antibody was det...

Objective. To study the concentration of pro-inflammatory cytokine interleukin-17 in blood serum patients with multiple sclerosis.
 Materials and methods. 68 sclerosis were examined. The severity neurological deficit was assessed by EDSS MSFC. determined method enzyme-linked immunosorbent assay.
 Results. It found that sclerosis, serum, normal group as a whole, induced effect immunomo...

The aim of the present study was to determine the protective action of silymarin on acute toxicity due totetracycline severe overdose in cats. Thirty healthy cats were randomly allotted into five equal groups. Catsin group A were given tetracycline (single dose 120 mg⁄kg, p.o.); group B consisted of cats that receivedsilymarin (single dose 30 mg⁄kg, p.o.) concurrent with tetracycline administra...