The ESCRT machinery mediates reverse membrane scission. By quantitative fluorescence lattice light-sheet microscopy, we have shown that ESCRT-III subunits polymerize rapidly on yeast endosomes, together with the recruitment of at least two Vps4 hexamers. During their 3-45 s lifetimes, the ESCRT-III assemblies accumulated 75-200 Snf7 and 15-50 Vps24 molecules. Productive budding events required ...

Abstract The accurate description of friction is critical in the finite element (FE) simulation sheet metal forming process. Usually, oversimplified through use a constant Coulomb coefficient. In this study, application an existing multiscale model extended to hot stamping accounts for effects tool and surface topography as well evolution contact pressure, temperature, bulk strain during stampi...

In search for new manufacturing process, Single Point Incremental Forming (SPIF) emerged to be one of the useful processes for forming complicated shapes. This is a flexible forming process which eliminates the die, punch and errors due to them. It is widely used for batch production of different shapes which has applications in diverse fields like automobile, aerospace, medical industry, packa...

General notes: Present your solutions neatly. Do not turn in rough unreadable worksheets learn to take pride in your presentation. Show the relevant steps, so that partial points can be awarded. BOX your final answers where applicable. Draw figures wherever necessary. Please print out this question sheet and staple to the top of your homework. Write your name and email address on the cover. The...

Stamping simulations usually make the plane stress simplifying assumption. However, this becomes less valid when material draws around features with radius to sheet thickness ratios less than 20. Pereira, Yan & Rolfe (Wear, Vol.265, p.1687 (2008)) predicted that out-of-plane stress equivalent to material yield can occur because a line contact forms briefly at the start of the draw process. The ...

This study aimed to develop new statistical models for evaluating the specific ampere draw (SI) based on rock brittleness index in rock sawing process. A variety of rocks, including carbonate and granite, were cut by a fully instrumented laboratory-sawing rig with two different types of circular diamond saws. Laboratory tests were performed at different depths of cut and feed rates. Multiple cu...

ultrasonic vibration assisted single point incremental forming (uvaspif) process is an attractive and adaptive method in which a sheet metal is gradually and locally formed by a vibrating hemispherical-head tool. the ultrasonic excitation of forming tool reduces the average of vertical component of forming force and spring-back rate of the formed sample. the spring-back phenomenon is one of the...

Aerospace industry is a value-added and technology integrated industry. Sheet metal parts are important portion of aerostructures. The sheet metal production line combines five different routing types together. This study tries to make a comparison between job shop layout and cellular layout with lean concept. According to precedence diagram of sheet metal fabrication process, the model and mea...

In this paper, we draw a link between cortical intrinsic curvature and the distributions of tangential connection lengths. We suggest that differential rates of surface expansion not only lead to intrinsic curvature of the cortical sheet, but also to differential inter-neuronal spacing. We propose that there follows a consequential change in the profile of neuronal connections: specifically an ...

plastic instability which results in the localization and failure of sheet metals has been studied in equibiaxial stretching of commercial purity aluminium sheet. attention was focussed on two main industrial conditions of the material. these were representative of the materials that could be processed by the commercial batchannealing process (slow heating, long time) or by a continuous anneali...