نتایج جستجو برای: single strand conformation polymorphism sscp

تعداد نتایج: 995449  

Journal: :Journal of clinical microbiology 2001
B J Kim K H Lee B N Park S J Kim E M Park Y G Park G H Bai Y H Kook

Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical s...

Background: Tumor necrosis factor- a (TNF-α) is a cytokine that was identified as a factor with a wide range of proinflammatory activities. The expression of bovine TNF-α in mammary tissue during pregnancy seems to have a role in development of the corresponding glands.Objective: Single nucleotide polymorphisms (SNPs) were defined in 3′-flanking region of...

Journal: :Arquivos brasileiros de endocrinologia e metabologia 2007
Marcelo A C G dos Santos Elisangela Pereira de S Quedas Rodrigo de Almeida Toledo Delmar M Lourenço Sergio Pereira de A Toledo

Multiple endocrine neoplasia type 2 (MEN2) is an autosomal dominant inherited tumor syndrome caused by RET proto-oncogene germline mutations (RET). Here we tested the Conformation Sensitive Gel Electrophoresis (CSGE) as a screening method for RET hot-spot mutations. Seven MEN2 families were studied by direct sequencing analysis, CSGE and Single Strand Conformational Polymorphism (SSCP). Using C...

Journal: :international journal of reproductive biomedicine 0
zohreh hojati fatemeh nouri emamzadeh fariba dehghanian

background: some dynamic changes occurs during spermatogenesis such as histone removal and its replacement with transition nuclear protein and protamine. these proteins are required for packing and condensation of sperm chromatin. jhdm2a is a histone demethylase that directly binds to promoter regions of tnp1 and prm1 genes and controls their expression by removing h3k9 at their promoters.objec...

Elaheh Soleimanpour, Mohammad Hossein Nasr-Esfahani, Seyed-Morteza Javadirad, Zohreh Hojati,

Background: KDM3A is a key epigenetic regulator that is expressed in the testis and is required for packaging and condensation of sperm chromatin. To this point, the association of the KDM3A gene and infertility has not been studied in human. The aim of this study was to screen any new mutation in KDM3A gene to explore more details of human male infertility. Methods: In this work, 150 infertile...

Journal: :Journal of animal science 1999
A Barroso S Dunner J Cañón

We have optimized the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique to screen the most frequent variants (A1, A2, A3, and B) of the bovine beta-casein gene. Five partly overlapping PCR products (233, 234, 265, 466, and 498 bp) of Exon VII of the beta-casein gene that encompass the target point mutations were heat-denatured, separated on nondenaturing pol...

Journal: :Asian Pacific journal of cancer prevention : APJCP 2010
Sheikh Alina Bashir Arshad Ahmad Pandith Adfar Yousuf Nighat Parveen Mushtaq Ahmad Siddiqi Syed Muddassar Khursheed Iqbal Andrabi Malik Zainul Abdin

BACKGROUND AND AIM The focus of the study was to investigate the frequencies of homozygous deletions and mutations of p16 gene in gastric carcinomas in the Kashmiri population. METHODS A total of 84 gastric carcinoma patients were screened by the single strand conformation polymorphism (SSCP) technique and later by DNA sequencing to detect mutations of the p16 gene. Also PCR was applied furth...

A. Javanmard, A. Niazi B. Arefnejad, M.J. Zamiri Y. Mehdizadeh

BMP15 and GDF9 are two oogenesis specific genes play a pivotal role in female fertility in mammals and potential for improvement of prolificacy in marker-assisted selection. The aim of present research was to investigate the variation and association between BMP15 and GDF9 polymorphism and litter size in Markhoz goats. The sequence variability of the different amplified fragments utilized for g...

Journal: :Journal of animal science 1998
A Barroso S Dunner J Cañón

We used polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to screen the most frequent variants (A, B, C, and E) found in the bovine kappa-casein gene. The PCR products (453 bp) were heat-denatured, loaded onto nondenaturing polyacrylamide gels, and silver-stained. Each variant yielded patterns clearly distinguishable from the others. Optimal conditions for th...

Journal: :Clinical molecular pathology 1996
R Bertorelle G Esposito C Belluco L Bonaldi A Del Mistro D Nitti M Lise L Chieco-Bianchi

Aim-To correlate immunohistochemical staining with single strand conformation polymorphism (SSCP) analysis of the p53 gene in colorectal cancer in order to understand how the findings provided by the two techniques complement each other in defining p53 functional status.Methods-Frozen tumour tissue from 94 patients with colorectal cancer was studied for p53 protein accumulation and gene mutatio...

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