The functionalization of gold nanoparticles with DNA has been studied extensively in solution; however, these ensemble measurements do not reveal particle-to-particle differences. Here we study the functionalization of gold nanorods with thiolated single-stranded DNA (ssDNA) at the single-particle level. We exploit the sensitivity of the plasmon resonance to the local refractive index to study ...

Summary Single-stranded (ss) DNA nanostructures (DNs), created by unimolecular folding of kilo-based ssDNA, represent a new class constructs suitable for biomaterial and biomedical applications. Here, using DNA-cleaving enzymes (deoxyribozymes), we present strategy the efficient cost-effective production milligrams ssDNs. We designed "genes" ssDNs flanked self-cleaving deoxyribozymes a...

The deoxyribonucleic acid (DNA) polymerase(s) of Rous sarcoma virus synthesizes two principal products-single-stranded DNA in the form of a DNA:ribonucleic acid (RNA) hybrid and double-stranded DNA. All of the single-stranded product and 50% of the double-stranded product can be hybridized to 70S viral RNA. These results, in combination with data obtained by analysis of the kinetics of double-s...

We have analysed DNA from African cassava mosaic virus (ACMV)-infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and detected ACMV-specific DNAs by blot-hybridisation. ACMV DNA forms including the previously characterised single-stranded, open-circular, linear and supercoiled DNAs along with five previously uncharacterised heterogeneous DNAs (H1-H5) were resolved. The...

A proteolytic fragment of recA protein, missing about 15% of the protein at the C terminus, was found to promote assimilation of homologous single-stranded DNA into duplex DNA more efficiently than intact recA protein. This difference was not found if Escherichia coli single-stranded DNA binding protein was present. The ATPase activity of both intact recA protein and the fragment was identical....

Homologous recombination is essential for the accurate repair of double-stranded DNA breaks (DSBs). Initially, RecBCD complex resects ends DSB into 3′ single-stranded on which a RecA filament assembles. Next, locates homologous template sister chromosome. Here we directly visualize DSBs in single cells, using high-throughput microfluidics and fluorescence microscopy. We find that, Escherichia c...