نتایج جستجو برای: size exclusion chromatography sec

تعداد نتایج: 751939  

2015
Seong-Jun Park Hee-Sung Ahn Jun Seok Kim Cheolju Lee John Matthew Koomen

Eight aminoacyl-tRNA synthetases (M, K, Q, D, R, I, EP and LARS) and three auxiliary proteins (AIMP1, 2 and 3) are known to form a multi-tRNA synthetase complex (MSC) in mammalian cells. We combined size exclusion chromatography (SEC) with reversed-phase liquid chromatography multiple reaction monitoring mass spectrometry (RPLC-MRM-MS) to characterize MSC components and free ARS proteins in hum...

2003

Proteins are the essence of life processes. DNA contains the coded information for life, and is analogous to computer software, but it is the proteins that are analogous to the hardware, that actually carry out the job. Proteins have many roles, from catalysts (enzymes), through proteins that bind and interact with other molecules to control their behaviour, to structural and storage proteins w...

Journal: :BMC Biotechnology 2005
Tristan Ruysschaert Audrey Marque Jean-Luc Duteyrat Sylviane Lesieur Mathias Winterhalter Didier Fournier

BACKGROUND Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It has been speculated that lipid binds to the column material or the entire lipos...

Journal: :Acta Crystallographica Section A Foundations and Advances 2017

Journal: :Current Protocols in Nucleic Acid Chemistry 2011

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