The tSNARE SNAP-25 is expressed in pancreatic (beta)-cells and is involved in the regulated release of insulin. It has been shown previously that SNAP-25 associates with the plasma membrane consequent to palmitoylation of one or more cysteines in the central region of the molecule. The importance of palmitolyation in the biological function of SNAP-25 in exocytosis was not addressed. Furthermor...

BACKGROUND Bronchopulmonary dysplasia (BPD) is the most common serious pulmonary morbidity in premature infants. The score for neonatal acute physiology (SNAP) is a physiologic severity index for neonatal intensive care and correlates well with neonatal mortality and clinical outcomes. The prognostic value of the SNAP score for BPD in preterm infants remains to be clarified. The aim of the stud...

Reversible phosphorylation of neuronal proteins plays an important role in the regulation of neurotransmitter release. Myosin phosphatase holoenzyme (MP) consists of a protein phosphatase-1 (PP1) catalytic subunit (PP1c) and a regulatory subunit, termed myosin phosphatase targeting subunit (MYPT1). The primary function of MP is to regulate the phosphorylation level of contractile proteins; howe...

European corn borer, Ostrinia nubilalis (Hübner), is a major pest of processing snap bean because larvae are contaminants in pods. The incidence of O. nubilalis-contaminated beans has become uncommon in New York, possibly because widespread adoption of Bt field corn has suppressed populations. Snap bean fields located where Bt corn has been intensively grown in space and time may be at lower ri...

Structural similarity between proteins gives us insights into their evolutionary relationships when there is low sequence similarity. In this paper, we present a novel approach called SNAP for non-sequential pair-wise structural alignment. Starting from an initial alignment, our approach iterates over a two-step process consisting of a superposition step and an alignment step, until convergence...

Although the Venus flytrap (Dionaea muscipula) can be considered as one of the most extensively investigated carnivorous plants, knowledge is still scarce about diversity of the snap-trap motion, the functionality of snap traps under varying environmental conditions, and their opening motion. By conducting simple snap-trap closure experiments in air and under water, we present striking evidence...

Bronk P, Deák F, Wilson MC, Liu X, Südhof TC, Kavalali ET. Differential effects of SNAP-25 deletion on Ca -dependent and Ca -independent neurotransmission. J Neurophysiol 98: 794–806, 2007. First published June 6, 2007; doi:10.1152/jn.00226.2007. At the synapse, SNAP-25, along with syntaxin/HPC-1 and synaptobrevin/ VAMP, forms SNARE N-ethylmaleimide-sensitive factor [soluble (NSF) attachment pr...

The objective of this study was to determine lactation performance by dairy cows fed corn snaplage either alone (SNAP) or in combination with ground dry shelled corn (SPDC) versus rolled high-moisture shelled corn (RHMC). Dry-matter content was greater for RHMC (78.2%) than SNAP (68.5%). The starch content of SNAP was approximately 10%-units lower than RHMC. Sixty Holstein cows (30 primiparous ...

Synaptosome-associated protein of 25 kDa (SNAP-25) has been shown to play an important role in Ca2+-dependent exocytosis in neurons and endocrine cells. During fertilization, sperm-egg fusion induces cytosolic Ca2+mobilization and subsequently Ca2+-dependent cortical granule (CG) exocytosis in eggs. However, it is not yet clear whether SNAP-25 is involved in this process. In this study, we dete...

Extracellular renal interstitial (RI) cGMP modulates NO- and pressure-induced natriuresis in vivo in the rat. The present study objective was to test the hypothesis that an intact microtubulin network is required for transport of cGMP from intracellular sites into the extracellular compartment in vivo and that this transport is required for natriuresis induced by NO and increased renal perfusio...