Genetic association studies usually apply the simple chi-square (χ2)-test for testing between a single-nucleotide polymorphism (SNP) and particular phenotype, assuming genotypes phenotypes are independent. So, conventional χ2-test does not consider increased risk of an individual carrying increasing number disease responsible allele (a genotype). But, tests should be performed with consideratio...

myostatin (mstn), also known as growth and differentiation factor-8 (gdf8), is a member of the transforming growth factor beta (tgf-?) superfamily that decreases growth in mammals. a single nucleotide polymorphism (snp) in the myostatin (gdf8) 3'utr of oar2 has been known as responsible for muscular hypertrophy in foreign breeds. in this study, in order to identify single nucleotide polymo...

Mimosa bimucronata (Candolle) O. Kuntze, native to the American tropics, is worst invasive tree species in China, where it distributed southern coastal areas of Guangdong and Fujian provinces. It characterized by rapid growth waterlogging resistance, was introduced China for slope bank protection. However, there still a lack effective molecular markers its invasion management. In this study, we...

Background: Type 1 diabetes mellitus (T1DM) is a chronic, immune-mediated disease characterized by the destruction of insulin-producing β cells in pancreas. This study investigated influence single nucleotide polymorphisms (SNP) tumor necrosis factor (TNF-α) –308 G/A (rs1800629,) on T1DM patients. Method: The was included 75 patients and 25 healthy subjects. SNP–308 TNF-α gene detected using po...

OBJECTIVE To examine the role of calpain-10 SNP-44, -43, -63 and del/ins-19 in genetic susceptibility to type 2 diabetes mellitus (T2DM) and associations with triglycerides and total cholesterol in a group of subjects residing in the Gaza Strip. SUBJECTS AND METHODS Ninety-six individuals were examined: 48 T2DM patients and 48 controls. The groups were genotyped for calpain-10 SNP-44, -43, -6...

A novel chip-based detection approach for single-nucleotide polymorphism (SNP) typing based on nanoparticle-coupled DNA-templated ligation reactions is reported. In contrast to conventional methods or recently developed techniques, this approach does not need costly instrumentation and complex stringency washing processes and offers both rapid multiplex SNP detection capability and ultrahigh se...

Best linear unbiased prediction (BLUP) has been used to estimate the fixed effects and random effects of complex traits. Traditionally, genomic relationship matrix-based (GRM) and random marker-based BLUP analyses are prevalent to estimate the genetic values of complex traits. We used three methods: GRM-based prediction (G-BLUP), random marker-based prediction using an identity matrix (so-calle...

Single nucleotide polymorphisms (SNPs) can be genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Before performing PCR-RFLP for SNP genotyping, a feasible primer pair observes numerous constraints and an available restriction enzyme discriminate the target SNP, are required, here we called it natural SNP-RFLP primer design. Design the natural SNP-RFLP pr...

results our study showed that there were no statistically significant differences in the allele and genotype frequencies of -1661 a/g snp between patients with sle and healthy controls (p > 0.05). conclusions in conclusion, we found no association between snp-1661 a/g of the ctla-4 promoter region and susceptibility to sle in our population. objectives to evaluate the potential influences of co...

INTRODUCTION Interleukin-18 (IL-18) is a Th1 cytokine, which is postulated to play a role in systemic lupus erythematosus (SLE). Two single nucleotide polymorphisms (SNPs) in the IL-18 promoter gene region were found to influence the quantitative expression of the IL-18 protein. The aim of this study was to determine whether IL-18 promoter gene polymorphisms are associated with SLE. MATERIALS...