The performance of different chemometric approaches was evaluated in the spectrophotometric determination of pharmaceutical mixtures characterized by having the amount of components with a very high ratio. Principal component regression (PCR), partial least squares with one dependent variable (PLS1) or multi-dependent variables (PLS2), and multivariate curve resolution (MCR) were applied to the...

The metabolic fate of a p.o. dose of 3.5 mmol 15N-labeled nitrate has been investigated in 12 healthy young adults. Samples of urine, saliva, plasma, and feces were collected over a period of 48 hr following administration of the dose. Subjects received either 60 mg of ascorbic acid, 2 g of ascorbic acid, or 2 g of sodium ascorbate per day. An average of 60% of the 15NO3- dose appeared in the u...

BACKGROUND Inhibitors of hyaluronidase are potent agents that maintain hyaluronic acid homeostasis and may serve as anti-aging, anti-inflammatory, and anti-microbial agents. Sodium copper chlorophyllin complex is being used therapeutically as a component in anti-aging cosmeceuticals, and has been shown to have anti-hyaluronidase activity. In this study we evaluated various commercial lots of so...

We report here on the cloning and functional characterization of human SVCT2, a sodium-dependent vitamin C (ascorbate) transporter. The hSVCT2 cDNA obtained from a human placental choriocarcinoma cell cDNA library, codes for a protein of 650 amino acids with a predicted molecular mass of 70 kDa. At the level of amino acid sequence, the human SVCT2 exhibits 95% identity to its rat homolog. When ...

We have studied the effect of ascorbic acid on voltage-dependent calcium channels in pancreatic beta cells. Using the whole-cell and perforated-patch variants of the patch clamp technique to record calcium tail currents, we have shown that the slowly deactivating (SD) calcium channel, which is similar to the T-type channel in other cells, is inhibited in a voltage-dependent manner by ascorbic a...

Elevated low-density lipoproteins (LDL) are associated with cutaneous microvascular dysfunction partially mediated by increased arginase activity, which is decreased following a systemic atorvastatin therapy. We hypothesized that increased ascorbate-sensitive oxidant stress, partially mediated through uncoupled nitric oxide synthase (NOS) induced by upregulated arginase, contributes to cutaneou...

AIM The aim of this study was to compare the neutralizing effect of antioxidant agents on the microleakage of dental restorations in nonvital teeth after bleaching with carbamide peroxide. Also assessed was the correlation of the microleakage data between an electrochemical and a staining technique. METHODS AND MATERIALS Following root canal therapy, 40 sound and similar human maxillary centr...

AIM The goal of this study was to fnd a method to eliminate bond strength reduction after vital bleaching. MATERIALS AND METHODS Sixty fat surfaces of enamel central human incisors were prepared by diamond disks. In the frst group, the samples immediately bonded to the tooth after bleaching. For the second group, the bleached samples were treated by sodium ascorbate for 1 hours. In the third ...