The plasma membrane ATPase of Neurospom cmsa, which is believed to function as an electrogenic proton pump, is extremely sensitive to inhibition by vanadium in the +5 oxidation state (vanadate). At pH 6.7 (the pH optimum of the ATPase), inhibition is half-maximal between 0.45 and 1.0 PM vanadate, depending upon the ionic composition of the reaction medium. Inhibition also depends upon pH, with ...

The behaviour of Mg related to vanadium(V)-induced lipid peroxidation (LPO) under in vitro conditions was examined. The studies performed on the liver supernatants (LS) obtained from control, sodium metavanadate-intoxicated, and sodium metavanadate-magnesium sulphate-administered male Wistar rats revealed and confirmed the pro-oxidative potential of V. Simultaneously, they indicated that the im...

It has been shown that the combination of benzylamine or tyramine and low concentrations of vanadate markedly stimulates glucose transport in rat adipocytes by a mechanism that requires semicarbazide-sensitive amine oxidase (SSAO) activity and H(2)O(2) formation. Here we have further analysed the insulin-like effects of the combination of SSAO substrates and vanadate and we have studied the sig...

The plasma membrane ATPase of Neurospom cmsa, which is believed to function as an electrogenic proton pump, is extremely sensitive to inhibition by vanadium in the +5 oxidation state (vanadate). At pH 6.7 (the pH optimum of the ATPase), inhibition is half-maximal between 0.45 and 1.0 PM vanadate, depending upon the ionic composition of the reaction medium. Inhibition also depends upon pH, with ...

Ca2+ + Mg2+-dependent ATPase from sarcoplasmic reticulum was inhibited by preincubation with vanadate. When the inhibited enzyme was preincubated in the presence of vanadate and assayed in its absence, a slow reactivation process was observed. This slow, hysteretic, process was exploited to study the influence of Ca2+ and ATP on the dissociation of vanadate. Ca2+ alone slowly displaced vanadate...

Transition metal oxyanions, such as molybdate, tungstate and vandadate, have been shown to prevent in vitro hormone-induced activation of the glucocorticoid receptor (GR) by blocking dissociation of the GR/heat shock protein heterocomplex. In this work, we report a novel effect of vanadate: in vivo potentiation of GR-mediated gene expression. In cells stably-transfected with complex (mouse mamm...

The interaction of vanadate with the (Na' + K')st imulated ATPase from electric organ was investigated using the acid quench-flow technique. A t 21"C, incubation of the enzyme with 1.3 to 1.6 p~ vanadate in the presence of 75 m~ Na' and 25 mM K' strongly inhibits phosphorylation by ATP. Enzyme activity remaining under these conditions shows no change in the apparent rates of phosphorylation or...

Semicarbazide-sensitive amine oxidase (SSAO) is highly expressed in adipose cells, and substrates of SSAO, such as benzylamine, in combination with low concentrations of vanadate strongly stimulate glucose transport and GLUT4 recruitment in 3T3-L1 and rat adipocytes. Here we examined whether acute and chronic administration of benzylamine and vanadate in vivo enhances glucose tolerance and redu...

The properties of the basal Ca2+ influx (measured using cells labelled with 45Ca2+) in intact human red blood cells (RBC) were compared with those of 45Ca2+ influx induced by vanadate. The basal Ca2+ influx was not sensitive to inhibitors of vanadate-induced Ca2+ influx such as the HS-reagent p-chloromercuribenzoate and low concentrations of Cu2+, and also the sensitivity to nifedipine was sign...

The formation of transition state mimics of phosphoryl transfer reactions with the metal oxoanion vanadate is a powerful technique in macromolecular crystallography. The tendency of vanadate to form pentacovalent complexes exhibiting trigonal bipyramidal geometry makes this compound a close approximation of the transition state for such reactions. In many cases, vanadate complexes provide the m...