Mitigation of ethylene promoted leaf senescence by lysophosphatidylethanolamine (LPE) was studied. Micropropagated ‘Russet Burbank’ potato (Solanum tuberosum L.,) plantlets were grown on MS media in sterile culture tubes. After 2 weeks of growth, tubes were sealed and ethylene gas was applied to obtain 5 nL·L fi nal concentration in the culture tubes. Observations and measurements were taken tw...

The kinetics of full-length and PH domain truncated cloned PI-PLC δ1 from rat toward soluble substrates [inositol 1,2-(cyclic)-phosphate (cIP) and glycerophosphoinositol phosphates (GPIPx)] as well as PI in detergent micelles provide the following insights into the mechanism of this enzyme. (i) That cIP is a substrate for the enzyme implies a two-step mechanism for PI hydrolysis [intramolecular...

Open AccessCCS ChemistryRESEARCH ARTICLE1 Nov 2021The Mechanochemical Release of Naphthalimide Fluorophores from ?-Carbonate and ?-Carbamate Disulfide-Centered Polymers Zhiyuan Shi, Qingchuan Song, Robert Göstl Andreas Herrmann Shi DWI—Leibniz Institute for Interactive Materials, Aachen 52056 Technical Macromolecular Chemistry, RWTH University, 52074 Department I Internal Medicine, University H...

In light of the potential use of the thiazolidinedione family of peroxisome proliferator-activated receptor(PPAR ) agonists in prostate cancer treatment, this study assessed the mechanism by which these agents suppress prostate-specific antigen (PSA) secretion in prostate cancer cells. Two lines of evidence indicate that the effect of thiazolidinediones on PSA downregulation is independent of P...

Amyloid fibrils comprising primarily the peptides Aâ40 and Aâ42 are a defining feature of the Alzheimer’s disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of potential AD diagnostics and therapeutics, since the pathogenic entity is not necessari...

Sphingolipid breakdown products, including ceramide and sphingosine, regulate cell growth, cell differentiation, and apoptosis. We examined the effect of various agents, including sphingolipids, on apoptosis induction in human epidermoid carcinoma KB-3-1 and its multidrug-resistant (MDR) subclone KB-C2 cells which express P-glycoprotein. Adriamycin (ADM) induced apoptosis in KB-3-1 cells but no...

Phosphoinositide 3-kinases (PI3Ks) are a family of lipid kinases that are activated by growth factor and G-protein coupled receptors, and propagate intracellular signals for growth, survival, proliferation, and metabolism. p85α, a modular protein consisting of 5 domains, binds and inhibits the enzymatic activity of Class IA PI3Ks. Here, we describe the structural states of the p85α dimer, based...

We investigated the effect of adenovirally mediated overexpression of adenylyl cyclase type 6 (AC6), a major form of AC expressed in mammalian heart, on G protein-coupled receptor regulation of cAMP production in neonatal rat ventricular myocytes. Following gene transfer of AC6, isoproterenoland forskolin-stimulated increases in cAMP were markedly enhanced, whereas basal levels of cAMP and resp...

Triton X-100-insoluble residues, or skeletons, of plasma membrane-rich vesicles obtained from unstimulated human platelets were isolated by high speed centrifugation. About 10-15% of the total surface iodinatable glycoproteins lib and III (GPIIb and GPIII, respectively) co-isolated with the insoluble fraction. After sonication and centrifugation the solubilized material was further purified by ...

Accelerated methods for partitioning of Cd, Cr, Cu, Ni, Pb and Zn, such as the use of single and ultrasonic extractions are assessed in terms of readsorption and compared with the three-stage sequential extraction scheme (SES) of the Standards Measurements and Testing Programme (SM&T). The standard addition approach was employed for characterising the readsorption artefact by applying the above...