This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphomet...

AIM The present study was designed to determine the effect of a 4-month oral treatment with tablets of Lepidium meyenii (Maca) on seminal analysis in nine adult normal men aged 24-44 years old. METHODS Nine men received tablets of Maca (1500 or 3000 mg/day) for 4 months. Seminal analysis was performed according to guidelines of the World Health Organization (WHO). Serum luteinizing hormone (L...

Abstract Study question To evaluate an automated sperm morphology assessment method using the KNIME Analytics Platform compared to manual analysis performed as per WHO guidelines. Summary answer Our emphasizes how artificial intelligence technologies have potential foster standardization of with comparable precision and reliability. What is known already Manual considered most difficult paramet...

This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-a...

The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analys...

Assuring the highest possible quality of care to the patient is the first goal of the andrology laboratory. Quality control and quality assurance as they apply to the andrology laboratory are outlined, and special problems in quality control for sperm function assays are addressed. An example of the patient review process is presented. Quality of care also depends on the ability of the laborato...

The data on the role of consanguinity in male infertility are scarce. The presence of systematic sperm defects in consanguineous respect to non-consanguineous population was demonstrated. Systematic defects are characterized by a specific alteration present in sperm for the whole life of the carrier. This study was aimed at exploring the sperm quality in selected infertile consanguineous patien...

Background: This study investigated the probable effects of daily administration of methamphetamine (MA) on sperm motility, acrosome reaction (AR), acridine orange (AO), sperm chromatin dispersion (SCD) test and chromomycin A3 (CMA3) in mice. Materials and Methods: Twenty four NMRI male mice were administrated three different doses of MA intraperitoneally (n=6 in each group). Different doses of...

Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial a...

BACKGROUND Factors influencing success of sperm retrieval in azoospermic patients and outcome of ICSI were evaluated. METHODS AND RESULTS Uni- and multifactorial analysis were performed using logistic and stepwise analysis, following surgical sperm retrieval by percutaneous epididymal sperm aspiration (55 cycles) or testicular sperm extraction (142 cycles) in 52 and 123 patients with obstruct...