It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin d...

REČKOVá, Z., MACHATKOVá, M., RyBář, R., MáCHAL, L.: Monitoring of chromatin integrity changes in the population of motile bovine sperm capacitated in vitro. Acta univ. agric. et silvic. Mendel. Brun., 2007, LV, No. 2, pp. 65–70 The objective of our study was to standardize a method for chromatin integrity assessment in a separated population of bovine sperm and monitor the changes occurring dur...

Changes of chromosomal basic proteins of rats have been followed during transformation of spermatids into spermatozoa in the testis and during maturation of spermatozoa in the epididymis. Rat testis chromatin has been fractionated on the basis of differing sensitivity to shearing, yielding a soluble fraction and a condensed fraction. The sperm histone is found in the condensed fraction. Somatic...

The relationship between sperm nuclear shape and bull fertility was determined. Two groups of bulls, 3 per group, were selected. Bulls differed in fertility based on lifetime nonreturn rates. Digital images of propidium iodide-stained sperm from each bull were collected and shape-evaluated by Fourier harmonic amplitudes 0 to 5. A discriminant function (P < .05) was constructed based on harmonic...

Background The solid and colloidal particles in the range of 1-100 nm are called nanoparticles. Recently, one of the most important metal nanoparticles, with wide usage is gold nanoparticle. Based on the previous studies, gold nanoparticles have spermatotoxic effects and can destroy sperm motility. The aim of present study is the effect of gold nanoparticles on chromatin quality and sperm param...

Background: Varicocele occurs in approximately 15% to 20% of the male population and it is the most common cause of poor semen parameters. It has been demonstrated that patients with varicocele have poor chromatin packing, DNA damage and nuclear anomalies than the healthy men. Therefore, the aim of this study was to evaluate sperm chromatin integrity and semen parameters in these patients after...

OBJECTIVE The present study was designed to determine the effect of the freeze-thawing procedure, computer controlled rate freezing and duration for six months, on human sperm chromatin (assessed by acridine orange test), vitality, motility, and morphology. DESIGN Experimental study MATERIAL AND METHOD Twenty semen samples were obtained from patients attending the infertility unit. The seme...

Objective The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and D...

OBJECTIVE To examine the relationship among sperm apoptosis, sperm chromatin status, and DNA ploidy in different sperm fractions. DESIGN Prospective study. SETTING Reproductive research center in a tertiary care hospital. INTERVENTION(S) Sperm prepared by density gradient were evaluated for sperm count, motility, apoptosis, and sperm chromatin assessment. MAIN OUTCOME MEASURE(S) Sperm c...

side effects of ciprofloxacin (cpfx), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. in the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm dna damage using aniline blue and acridine orange technique, respectively. the fertility potential in male mice was also evaluated. nmri male...