OBJECTIVE To develop a simple rapid procedure for bioreduction of silver nanoparticles (AgNPs) using aqueous leaves extracts of Catharanthus roseus (C. roseus). METHODS Characterization were determined by using UV-Vis spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. RESULTS SEM showed the formation of silver nanoparticles with an average ...

an on-line uv-hplc / apci-ms (atmospheric pressure chemical ionization) tandem by a platinium c18 column with an isocratic solvent system after optimizing was applied to the separation and the identification of different carotenoids including astaxanthin, canthaxanthin, apocarotenoic ester, torularhodin and beta carotene. this method was used to identify particular carotenoids produced by newly...

The Catharanthus roseus L. plant leaf extracts were prepared by using solvents such as acetone, ethanol, and chloroform and tested against pathogenic microorganisms to determine their antimicrobial potential. Catharanthus roseus leaf extracts showed maximum antibacterial activity against all the pathogenic microorganisms. Among the solvents tested, ethanol showed maximum antibacterial activity ...

The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetic...

The mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than Candida being no exception. This is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging i...

The in vitro antifungal activity of a new triazole derivative, voriconazole, was compared with those of itraconazole and amphotericin B against 67 isolates of Aspergillus flavus, Aspergillus fumigatus, Bipolaris spp., Fusarium oxysporum, Fusarium solani, Pseudallescheria boydii, Rhizopus arrhizus, Blastomyces dermatitidis, Histoplasma capsulatum, and Sporothrix schenckii. The in vitro activitie...

Although “Prelog’s rule” and “two hydrophobic binding pockets” model have been used to predict and explain the stereoselectivity of enzymatic ketone reduction, the molecular basis of stereorecognition by carbonyl reductases has not been well understood. The stereo selectivity is not only determined by the structures of enzymes and substrates, but also affected by the reaction conditions such as...

The ability to identify yeast isolates by the new enzymatic RapID Yeast Plus System was compared to the ability to identify yeast isolates by the API 20C system. A total of 447 yeast isolates representing Blastoschizomyces capitatus, 17 Candida spp., 5 Cryptococcus spp., Geotrichum spp., 2 Hanseniaspora spp., Hansenula anomala, Hansenula wingei, 3 Rhodotorula spp., Saccharomyces cerevisiae, Spo...