Bacillus subtilis ATCC 6051 and ATCC 6633 spores used in bacterial inhibition screening assays for genetic metabolism defects in newborn infants were produced by using liquid synthetic replacement sporulation media. These media allowed a high degree of sporulation, as judged by direct cell counts. Sporulation took place within 23 to 27 h with these media. Also, a more rational procedure for sel...

Asthma is a global problem that affects over 300 million people in the world. The main reason of asthma is allergy and atopy. Asthmatic patients are sensitized to allergens that can be resulting asthma attacks. Fungi as an allergen can be sensitized atopic people and present a severe asthma. Fungi have been found in water-damaged homes of asthmatic patients. Allergic asthma is associated with e...

Bacillus subtilis sporulation is a developmental process that culminates in the formation of a highly resistant and persistent endospore. Inhibiting DNA synthesis prior to the completion of the final round of DNA replication blocks sporulation at an early stage. Conditions that prevent compartmentalization of gene expression, i.e. inhibition of asymmetric septum formation or chromosome partitio...

Clostridium difficile is the leading cause of antibiotic-associated diarrhoea and a major burden to healthcare services worldwide. In recent years, C. difficile strains belonging to the BI/NAP1/027 type have become highly represented among clinical isolates. These so-called 'hypervirulent' strains are associated with outbreaks of increased disease severity, higher relapse rates and an expanded ...

The study of the antagonistic activity eleven isolates Trichoderma spp., against four Botrytis cinerea, collected from diseased tomato plants cultivated in greenhouses located on Algiers coast and north-east Sahara. vitro antagonism was compared to fungicidal "PROLEX", widely used by farmers this phytopathogen Algeria. results revealed a complete inhibition sporulation, germination, production ...

A class of rifampin-resistant (rfm) mutations of Bacillus subtilis suppresses the temperature-sensitive sporulation of a fusidic acid-resistant mutant. FUS426, which has an altered elongation factor G. The rfm mutation suppressed only the sporulation defect caused by the elongation factor G mutation, but could not suppress other types of induced sporulation defects. Genetic and biochemical anal...

In facultatively sexual species, lineages that reproduce asexually for a period of time can accumulate mutations that reduce their ability to undergo sexual reproduction when sex is favorable. We propagated Saccharomyces cerevisiae asexually for approximately 800 generations, after which we measured the change in sexual fitness, measured as the proportion of asci observed in sporulation medium....

Entry into sporulation is governed by the master regulator Spo0A. Spo0A accumulates in its active form, Spo0A-P, as cells enter stationary phase. Prior reports have shown that the acute induction of constitutively active Spo0A during exponential growth does not result in sporulation. However, a subsequent study also found that a gradual increase in Spo0A-P, mediated through artificial expressio...

Clostridium perfringens type A strains producing enterotoxin (CPE) cause one of the most common bacterial food-borne illnesses, as well as many cases of non-food-borne human gastrointestinal disease. Recent studies have shown that an Agr-like quorum-sensing system controls production of chromosomally encoded alpha-toxin and perfringolysin O by C. perfringens, as well as sporulation by Clostridi...

A plasmid, pSAl.1, of StrePtomyces a.zuyeus elicited conjugative pocks and inhibited the sporulation of its host mycelia. The analysis of plasmid pSA1.1 was hampered by the lack of selective marker. Therefore, the introduction of a kanamycin resistance gene (Fzmr) derived from Strefltomyces kanamyceticus to pSA1.1 was tried. The obtained recombinant still preserved the sporulation-inhibitory an...