Background Spermatogonial stem cells (SSCs) are undifferentiated cells which are highly reproducible and expandable. Several studies have been conducted to reproduce these cells in culture. They used growth factors, hormones and different feeder cells to improve survival and proliferation of SSCs. Objective This study was conducted to evaluate the effects of follicular stimulating hormone (FS...

Proper regulation of spermatogonial stem cells (SSCs) is crucial for sustaining steady-state spermatogenesis. Previous work has identified several paracrine factors involved in this regulation, in particular, glial cell line-derived neurotrophic factor and fibroblast growth factor 2, which promote long-term SSC self-renewal. Using a SSC culture system, we have recently reported that Wnt5a promo...

Spermatogonial stem cells (SSCs) are a self-renewing population of adult stem cells capable of producing progeny cells for sperm production throughout the life of the male. Regulation of the SSC population includes establishment and maintenance of a niche microenvironment in the seminiferous tubules of the testis. Signaling from somatic cells within the niche determines the fate of SSCs by eith...

background: niche cells, regulating spermatogonial stem cells (sscs) fate are believed to have a reciprocal communication with sscs. the present study was conducted to evaluate the effect of ssc elimination on the gene expression of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor 2 (fgf2) and kit ligand (kitlg), which are the main growth factors regulating sscs deve...

BACKGROUND It has been evidenced in the literature that exposure to cigarette smoke causes hypertension in rats; however, it has not been demonstrated if the baroreflex function is impaired before the animal becomes hypertensive. OBJECTIVE We evaluated short-term effects of sidestream cigarette smoke (SSCS) exposure on baroreflex function in Wistar normotensive rats. METHODS Rats were expos...

Pathogenic de novo mutations increase with fathers' age and could be amplified through competition between genetically distinct subpopulations of spermatogonial stem cells (SSCs). Here, we tested the fitness of SSCs bearing wild-type human FGFR2 or an Apert syndrome mutant, FGFR2 (S252W), to provide experimental evidence for SSC competition. The S252W allele conferred enhanced FGFR2-mediated si...

Emerging evidence indicates that somatic stem cells (SSCs) of different types prominently contribute to endometrium-associated disorders such as endometriosis. We reviewed the pertinent studies available on PubMed, published in English language until December 2014 and focused on the involvement of SSCs in the pathogenesis of this common gynecological disease. A concise summary of the data obtai...

The dynamical evolution of super star clusters (SSCs) moving in the background of dark matter halo has been investigated as a possible event causing the observed correlation between the mass of galactic bulge, Mbulge, and the mass of its central black hole, MBH . The involved physical processes are the sinking of SSCs due to the dynamical friction, and the stripping of SSCs on their way to the ...

Men with testicular failure, either primary or secondary, have been shown to be interested in fertility preservation. Spermatogonial stem cell (SSC) transplantation is currently being investigated as a treatment for this. Currently this experimental technique consists of cryopreservation of a testicular biopsy prior to cancer treatment, followed by optional in vitro expansion of SSCs and auto t...

BACKGROUND To isolate and characterization of human spermatogonial stem cells from stem spermatogonium. METHODS The disassociation of spermatogonial stem cells (SSCs) were performed using enzymatic digestion of type I collagenase and trypsin. The SSCs were isolated by using Percoll density gradient centrifugation, followed by differential surface-attachment method. Octamer-4(OCT4)-positive SS...