Aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylases (16S RMTase) are two main resistance mechanisms against aminoglycosides. This study aimed to evaluate the frequency of AMEs methylase genes among aminoglycoside non-susceptible Acinetobacter baumannii isolates assess their clonal relationship using repetitive extragenic palindromic-PCR (rep-PCR). In this cross-sectional study, a to...

Purpose: Prove that Computed Tomography (CT) is a more effective diagnostic tool for diagnosing Coronavirus Disease 2019 (COVID-19) in comparison with Reverse Transcription Polymerase Chain Reaction (RT-PCR) due to its higher sensitivity.
 Methods and materials: A total of 200 cases had records chest CT scans RT-PCR tests were collected from Palestinian governmental hospitals September Nov...

Polymerase Chain Reaction (PCR) merupakan uji diagnostik yang sangat sensitif. Enzim Taq salah satu komponen paling penting dalam pengujian (PCR). taq polymerase harganya mahal. Penggunaan pada PCR kurang efisien. Untuk mengetahui efisiensi penggunaan enzim dan volume terkecil berapa masih dapat digunakan metode PCR, maka dilakukanlah penelitian dengan membuat lebih kecil (5µl, 10 µl, 25 µl) da...

introduction: differential diagnosis of potential pathogenic entamoeba histological from non-pathogenic entamoeba dispar by a molecular method. material and methods: one pair of primers designed based on sequence of genomic dna coding the 30kd surface antigen of e.histolytica/e.dispar. a 374 base pair pcr product was amplified by using the primers. electrophoretic patterns of pcr product digest...

AIMS To evaluate different hybridisation techniques to detect and type human papillomavirus (HPV) DNAs amplified by consensus primer polymerase chain reaction (PCR) in biopsy and cytological specimens. METHODS A hybrid capture-immunoassay in microtitre wells was performed to detect HPV sequences amplified by PCR and typed by specific oligoprobes. Consensus primers were used to amplify a seque...

The use of reverse transcription polymerase chain reaction (RT-PCR) with internal RNA competitive standards (competitors) provides a means for measuring absolute amounts of mRNA transcripts in small numbers of cells. Most quantitative competitive (QC)-RT-PCR methods require analysis of multiple reactions to determine the equimolar point of the products produced from mRNA vs. competitor RNA. Her...

The 16S rRNA gene has previously been used to develop genus-specific PCR primers for identification of enterococci. In addition, the superoxide dismutase gene (sodA) has been identified as a potential target for species differentiation of enterococci. In this study, Enterococcus genus-specific primers developed by Deasy et al. (E1/E2) were incorporated with species-specific primers based upon t...

BACKGROUND The urine dipstick is widely used as an initial screening tool for the evaluation of proteinuria; however, its diagnostic accuracy has not yet been sufficiently evaluated. Therefore, we evaluated its diagnostic accuracy using spot urine albumin/creatinine ratio (ACR) and total protein/creatinine ratio (PCR) in proteinuria. METHODS Using PCR ≥0.2 g/g or ≥0.5 g/g and ACR ≥300 mg/g or...

The protistan parasite Mikrocytos mackini, the causative agent of Denman Island disease in the oyster Crassostrea gigas in British Columbia, Canada, is of wide concern because it can infect other oyster species and because its life cycle, mode of transmission, and origins are unknown. PCR and fluorescent in situ hybridization (FISH) assays were developed for M. mackini, the PCR assay was valida...

To standardize a PCR assay for the detection of parvovirus B19 DNA in serum samples three different sample treatments were evaluated on the basis of the efficiency of recovery, reproducibility, convenience of sample handling, and presence of PCR inhibitors. Moreover, the presence of an internal standard competitor as the working reagent at one defined concentration in a competitive PCR-ELISA ha...